Issue 26, 2011
From the journal:

Chemical Communications

Quantitative label-free RNA detection using surface-enhanced Raman spectroscopy†

Enora Prado,ab   Nicolas Daugey,b   Sébastien Plumet,c   Laurent Servant*b  and  Sophie Lecomte*a  

* Corresponding authors

a Université Bordeaux 1—CNRS, Chimie et Biologie des Membranes et des Nanoobjets, 2 rue Robert Escarpit, 33607 Pessac, France
E-mail: s.lecomte@cbmn.u-bordeaux.fr
Tel: +33 (0)5 40 00 30 47

b Université Bordeaux 1—CNRS, Institut des Sciences Moléculaires, 351 cours de la libération, 33405 Talence, France
E-mail: l.servant@ism.u-bordeaux1.fr
Tel: +33 (0)540006416

c DGA/D4S/MRIS, 7, rue des Mathurins, 92250 Bagneux, France.

Abstract

Surface-Enhanced Raman Spectroscopy (SERS) was performed to detect label-free RNA. We defined conditions which make it possible to probe the four bases of RNA, in single strands of polyadenosine (pA), polyuridine (pU), polycytosine (pC) and polyguanosine (pG). We therefore present below a quantitative analysis of mixtures of non-hybridized single strands, based on the deconvolution of the SERS mixture spectrum into the relative contributions of the SERS spectra of each constituent.

Graphical abstract: Quantitative label-free RNA detection using surface-enhanced Raman spectroscopy

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C1CC11925G
Article type
Communication
Submitted
05 Apr 2011
Accepted
26 Apr 2011
First published
17 May 2011

Chem. Commun., 2011,47, 7425-7427

Permissions

Request permissions

Quantitative label-free RNA detection using surface-enhanced Raman spectroscopy

E. Prado, N. Daugey, S. Plumet, L. Servant and S. Lecomte, Chem. Commun., 2011, 47, 7425 DOI: 10.1039/C1CC11925G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements