Issue 17, 2011
From the journal:

Chemical Communications

Primer extension reactions as a tool to uncover folding motifs within complex G-rich sequences: analysis of the human KRASNHE†

Manikandan Paramasivam,‡a   Susanna Cogoia  and  Luigi E. Xodo*a  

* Corresponding authors

a Department of Medical and Biological Science, University of Udine, 33100 Udine, Italy
E-mail: luigi.xodo@uniud.it
Fax: +39 0432 494301
Tel: +39 0432 494395

Abstract

We employed primer extension reactions to uncover folding motifs in a nuclease hypersensitive element (NHE) with a complex guanine pattern, located in the human KRAS promoter. We also identified and characterized a new G-rich motif of 21 nt capable of forming a parallel G-quadruplex that is disrupted by protein UP1.

Graphical abstract: Primer extension reactions as a tool to uncover folding motifs within complex G-rich sequences: analysis of the human KRASNHE

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Article information

DOI
https://doi.org/10.1039/C1CC10269A
Article type
Communication
Submitted
14 Jan 2011
Accepted
21 Feb 2011
First published
22 Mar 2011

Chem. Commun., 2011,47, 4965-4967

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Primer extension reactions as a tool to uncover folding motifs within complex G-rich sequences: analysis of the human KRASNHE

M. Paramasivam, S. Cogoi and L. E. Xodo, Chem. Commun., 2011, 47, 4965 DOI: 10.1039/C1CC10269A

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