Issue 9, 2011
From the journal:

Analyst

Aptasensor based on the selective electrodeposition of protein-linked gold nanoparticles on screen-printed electrodes

M. Moreno,†*a   V. M. González,b   E. Rincón,a   A. Domingoa  and  E. Domínguezc  

* Corresponding authors

a Departamento Bioquímica y Biología Molecular, Facultad de Medicina, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain
E-mail: morenomm@cab.inta-csic.es
Fax: +34915201074
Tel: +34915206406

b Departamento de Bioquímica-Investigación, Hospital Ramón y Cajal (IRYCIS), Madrid, Spain

c Departamento Química Analítica e Ingeniería Química, Facultad de Farmacia, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain

Abstract

The electrodeposition of DNA–gold nanoparticles previously developed in our group has been used as starting point for the electrodeposition of proteins attached to gold nanoparticles. We have performed a proof of principle by developing a methodology based on the electrodeposition of proteins bound to gold nanoparticles on screen-printed gold microelectrodes using, in a first approach, horseradish peroxide-conjugated gold nanoparticles (gold–HRP). The electrodeposition was achieved at a current positive potential of 800 mV vs. Ag/AgCl and the functionality of the electrodeposited HRP-particles was tested by electrochemical reduction of H2O2. Furthermore, we used this proof of concept in an aptasensor application to detect Leishmania infantum KMP-11. Hence, we have demonstrated not only the functionality of the electrodeposition of proteins bound to gold nanoparticles, but also the utility of the method with the aim of developing a real biosensor containing multiple enzymes or proteins in a multimodular device.

Graphical abstract: Aptasensor based on the selective electrodeposition of protein-linked gold nanoparticles on screen-printed electrodes

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Article information

DOI
https://doi.org/10.1039/C1AN15070G
Article type
Communication
Submitted
25 Jan 2011
Accepted
23 Feb 2011
First published
11 Mar 2011

Analyst, 2011,136, 1810-1815

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Aptasensor based on the selective electrodeposition of protein-linked gold nanoparticles on screen-printed electrodes

M. Moreno, V. M. González, E. Rincón, A. Domingo and E. Domínguez, Analyst, 2011, 136, 1810 DOI: 10.1039/C1AN15070G

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