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Long-lived fluorescence of homopolymeric guanine—cytosine DNA duplexes

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Abstract

The fluorescence spectrum of the homopolymeric double helix poly(dG)·poly(dC) is dominated by emission decaying on the nanosecond time-scale, as previously reported for the alternating homologue poly(dGdC)·poly(dGdC). Thus, energy trapping over long periods of time is a common feature of GC duplexes which contrast with AT duplexes. The impact of such behaviour on DNA photodamage needs to be evaluated.

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Authors and Affiliations

  1. Laboratoire Francis Perrin, CEA/DSM/IRAMIS/SPAM - CNRS URA 2453, 91191, Gif-sur-Yvette, France

    Ignacio Vayá, Pascale Changenet-Barret, Thomas Gustavsson & Dimitra Markovitsi

  2. Department of Biochemistry, Georges S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, 69978, Israel

    Dragoslav Zikich & Alexander B. Kotlyar

Authors
  1. Ignacio Vayá
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  2. Pascale Changenet-Barret
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  3. Thomas Gustavsson
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  4. Dragoslav Zikich
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  5. Alexander B. Kotlyar
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  6. Dimitra Markovitsi
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Corresponding author

Correspondence to Dimitra Markovitsi.

Additional information

Electronic supplementary information (ESI) available: Synthesis and characterization of poly(dG)·poly(dC); details of spectroscopic measurements and fits of the fluorescence decays.

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Vayá, I., Changenet-Barret, P., Gustavsson, T. et al. Long-lived fluorescence of homopolymeric guanine—cytosine DNA duplexes. Photochem Photobiol Sci 9, 1193–1195 (2010). https://doi.org/10.1039/c0pp00201a

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  • DOI: https://doi.org/10.1039/c0pp00201a

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