Issue 9, 2010
From the journal:

Lab on a Chip

Horizontal nDEP cages within open microwell arrays for precise positioning of cells and particles†

Marta Lombardini,*a   Massimo Bocchi,*ab   Laura Rambelli,a   Luca Giulianellia  and  Roberto Guerrieria  

* Corresponding authors

a ARCES, University of Bologna, Viale Pepoli 3/2, Bologna, Italy
E-mail: marta.lombardini@unibo.it
Tel: +39 051 2093810

b Mindseeds Laboratories, Bologna, Italy
E-mail: massimo.bocchi@mindseedslabs.com
Tel: +39 051 2093815

Abstract

We present the structure of an open microwell, i.e. a microwell open at both the top and bottom ends, which enables single-cells to be handled, processed and recovered after the experiment. The microwell has a novel architecture which allows particles to be trapped and forced to interact by means of a cylindrical negative dielectrophoretic cage. Particles are aligned along a horizontal axis where the electric field minimum is placed. Arrays of open microwells are fabricated using flexible printed circuit board (PCB) technology providing cheap and disposable devices. Levitation and precise positioning of both polystyrene beads and K562 cells were experimented, confirming the results of physical simulations. Assessment of cell viability after 20 min exposure to the electric field was performed through a standard calcein-release assay.

Graphical abstract: Horizontal nDEP cages within open microwell arrays for precise positioning of cells and particles

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Article information

DOI
https://doi.org/10.1039/B923567A
Article type
Technical Note
Submitted
11 Nov 2009
Accepted
15 Jan 2010
First published
10 Feb 2010

Lab Chip, 2010,10, 1204-1207

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Horizontal nDEP cages within open microwell arrays for precise positioning of cells and particles

M. Lombardini, M. Bocchi, L. Rambelli, L. Giulianelli and R. Guerrieri, Lab Chip, 2010, 10, 1204 DOI: 10.1039/B923567A

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