Issue 47, 2009
From the journal:

Journal of Materials Chemistry

Capsules-in-bead scaffold: a rational architecture for spatially separated multienzyme cascade system

Yanjun Jiang,ab   Qianyun Sun,a   Lei Zhanga  and  Zhongyi Jiang*a  

* Corresponding authors

a Key Laboratory for Green Chemical Technology of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
E-mail: zhyjiang@tju.edu.cn

b School of Chemical Engineering and Technology, HeBei University of Technology, Tianjin 300130, China

Abstract

A novel kind of capsules-in-bead scaffold was developed to construct a spatially separated multienzyme cascade system. More specifically, each enzyme was first encapsulated in a guest microcapsule through the synergy of biomimetic mineralization and layer-by-layer self-assembly; these guest capsules were then encapsulated into a millimeter-scale gel bead (host bead). Such structured scaffold, was validated by the production of methanol from CO2via three dehydrogenases consisting of formate, formaldehyde, and alcohol dehydrogenases. Compared to the homogeneous multienzyme cascade system where enzymes were all co-encapsulated in a single gel bead, the capsules-in-bead scaffold substantially improved enzymatic activity, recycling and storage stability, thus higher methanol yield was achieved. Hopefully, this study will set an innovative example for the rational design of complex multienzyme cascades and multienzyme microreactor.

Graphical abstract: Capsules-in-bead scaffold: a rational architecture for spatially separated multienzyme cascade system

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Article information

DOI
https://doi.org/10.1039/B914268A
Article type
Paper
Submitted
16 Jul 2009
Accepted
28 Sep 2009
First published
22 Oct 2009

J. Mater. Chem., 2009,19, 9068-9074

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Capsules-in-bead scaffold: a rational architecture for spatially separated multienzyme cascade system

Y. Jiang, Q. Sun, L. Zhang and Z. Jiang, J. Mater. Chem., 2009, 19, 9068 DOI: 10.1039/B914268A

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