Abstract
Polyoxometalates (POMs) are useful in a wide range of biological applications, whilst rare-earth based POMs provide a potentially new biological optical label. As the luminescence of rare-earth materials is known to be sensitive to the environment, we report on investigations into the photophysics of a rare-earth (europium) POM with the protein bovine serum albumin (BSA). Via the use of luminescence anisotropy and time-resolved measurements the europium decatungstate was found to interact with BSA, which was accompanied by an observed enhancement in its luminescence.
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F. van de Rijke, H. Zijlmans, S. Li, T. Vail, A. K. Raap, R. S. Niedbala and H. J. Tanke, Up-converting phosphor reporters for nucleic acid microarrays, Nature Biotechol., 2001, 19, 273.
A. P. Alivisatos, W. Gu and C. Larabell, Quantum dots as cellular probes, Annu. Rev. Biomed. Eng., 2005, 7, 55.
M. Green, Semiconductor quantum dots as biological imaging agents, Angew. Chem., Int. Ed., 2004, 43, 4129.
K. A. Lidke, B. Rieger, T. M. Jovin and Rainer Heintzmann, Superres-olution by localization of quantum dots using blinking statistics, Opt. Express, 2005, 13, 7052.
A. M. Derfus, W. C. W. Chan and S. N. Bhatia, Probing the cytotoxicity of semiconductor quantum dots, Nano. Lett., 2004, 4, 11.
H. S. Choi, W. Liu, P. Misra, E. Tanaka, J. P. Zimmer, B. Kandapallil, M. G. Bawendi and J. V. Frangioni, Renal clearance of quantum dots, Nature Biotechol., 2007, 25, 1165.
J. L. Domingo, Vanadium and tungsten derivatives as antidiabetic agents - A review of their toxic effects, Biol. Trace Element Res., 2002, 88, 97.
I. Hemmilä and V. Laitala, Progress in lanthanides as luminescence probes, J. Fluoresc., 2005, 15, 529.
M. H. V. Werts, Making sense of lanthanide luminescence, Sci. Progress, 2005, 88, 101.
J. L. Kropp and M. W. Windsor, Luminescence and energy transfer in solutions of rare-earth complexes. I. Enhancement, of fluorescence by deuterium substitution, J. Chem. Phys., 1965, 42, 1599.
G. Zhang, B. Keita, C. T. Craescu, S. Miron, P. Oliveira and L. Nadjo, Polyoxometalate binding to human serum albumin: a thermodynamic and spectroscopic approach, J. Phys. Chem. B, 2007, 111, 11253.
K. Takehara, Y. Morinaga, S. Nakashima, S. Matsuoka, H. Kamaya and I. Ueda, Classification of the binding modes in bovine serum albumin using terminally substituted alkane analogues, Anal. Sci., 2006, 22, 1571.
D. Ajloo, H. Behnam, A. A. Saboury, F. Mohamadi-Zonoz, B. Ranjbar, A. A. Moosavi-Movahedi, Z. Hasani, K. Alizadeh, M. Gharanfoli and M. Amani, Thermodynamic and structural studies on the human serum albumin in the presence of a polyoxometalate, Bull. Korean Chem. Soc., 2007, 28, 730.
M. Green, J. Harries, G. Wakefield and R. Taylor, The synthesis of silica nanospheres doped with polyoxometalates, J. Am. Chem. Soc., 2005, 127, 12812.
M. Clemente-León, E. Coronado, A. Soriano-Portillo, C. Mingotaud and J. M. Domínguez-Vera, Langmuir-Blodgett films based on inorganic molecular complexes with magnetic or optical properties, Adv. Colloid Interface Sci., 2005, 116, 193.
T. Ito, H. Yashiro and T. Yamase, Two-dimensional photoluminescence behavior of Langmuir-Blodgett monolayers and multilayers composed of decatungstoeuropate, J. Cluster Sci., 2006, 17, 375.
M. Jiang, X. Zhai and M. Liu, Fabrication and photoluminescence of hybrid organized molecular films of a series of gemini amphiphiles and europium(III)-containing polyoxometalate, Langmuir, 2005, 21, 11128.
W. Bu, H. Li, W. Li, L. Wu, C. Zhai and Y. Wu, Surfactant-encapsulated europium-substituted heteropolyoxotungstates: structural characterizations and photophysical properties, J. Phys. Chem. B, 2004, 108, 12776.
G. Hungerford, M. Green and K. Suhling, Optical spectroscopy following the incorporation of a rare-earth containing (Eu) polyoxometalate into a sol-gel derived media, Phys. Chem. Chem. Phys., 2007, 9, 6012.
R. D. Peacock and T. J. R. Weakley, Heteropolytungstate complexes of lanthanide elements. 1. Preparation and reactions, J. Chem. Soc. A, 1971, 11, 1836.
L. Zhang, Y. Yao, X. Ye and Q. Wu, Luminescence behavior of Eu3+ in polytitanasiloxane solution, J. Polym. Sci., Part B: Polym. Phys., 2006, 44, 1357.
R. Ballardini, Q. G. Mulazzani, M. Venturi, F. Bolletta and V. Balzani, Photophysical characterization of the decatungstoeuropate(9-) anion, Inorg. Chem., 1984, 23, 300.
T. Yamase, T. Kobayashi, M. Sugeta and H. Naruke, Europium(III) luminescence and intramolecular energy transfer studies of polyox-ometalloeuropates, J. Phys. Chem. A, 1997, 101, 5046.
Calculated by taking the log of the difference in intensity compared to absence of BSA divided by the intensity and plotting this against the log [BSA]. A linear fit produced an association constant of 18 (from gradient) and number of sites at 0.4.
J. Hamblin, N. Abboyi and M. P. Lowe, A binaphthyl-containing Eu(III) complex and its interaction with human serum albumin: a luminescence study, Chem. Commun., 2005, 657.
G. Zhang, B. Keita, J.-C. Brochon, P. Oliveira, L. Nadjo, C. T. Craescu and S. Miron, Molecular interaction and energy transfer between human serum albumin and polyoxometalates, J. Phys. Chem. B, 2007, 111, 1809.
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Hungerford, G., Suhling, K. & Green, M. Luminescence enhancement of a europium containing polyoxometalate on interaction with bovine serum albumin. Photochem Photobiol Sci 7, 734–737 (2008). https://doi.org/10.1039/b802793e
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DOI: https://doi.org/10.1039/b802793e