Issue 6, 2007
From the journal:

Analyst

Emerging methods in proteomics: top-down protein characterization by multistage tandem mass spectrometry

Gwynyth Scherperela  and  Gavin E. Reid*ab  

* Corresponding authors

a Department of Chemistry, Michigan State University, 234 Chemistry Building, East Lansing, Michigan 48824, USA
E-mail: reid@chemistry.msu.edu
Fax: +1-(517)-353-1793.
Tel: +1-(517)-355-9715 ×198.

b Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824, USA

Abstract

‘Top-down’ mass spectrometry methods have emerged as an attractive alternative to conventional ‘bottom-up’ approaches for the comprehensive characterization of co- and post-translational protein modifications. Here we present a brief overview of current strategies employed for top-down protein characterization and discuss the key technical challenges and solutions associated with their implementation on a range of mass spectrometry instrument platforms. For more specific details regarding the individual strategies described herein, interested readers are referred to the references cited at the end of this article.

Graphical abstract: Emerging methods in proteomics: top-down protein characterization by multistage tandem mass spectrometry

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Article information

DOI
https://doi.org/10.1039/B618499P
Article type
Education
Submitted
18 Dec 2006
Accepted
28 Mar 2007
First published
10 Apr 2007

Analyst, 2007,132, 500-506

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Emerging methods in proteomics: top-down protein characterization by multistage tandem mass spectrometry

G. Scherperel and G. E. Reid, Analyst, 2007, 132, 500 DOI: 10.1039/B618499P

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