Issue 9, 2006

Two-dimensional impedance imaging of cell migration and epithelial stratification

Abstract

We present a miniaturized impedance imaging system, developed for 2D imaging of cell and tissue culture. The system is based on 16 microelectrodes (5 µm × 4 mm). An equivalent circuit for four-point (tetrapolar) impedance spectra was developed and validated. The system uses an Agilent 4294A impedance analyser combined with a front-end amplifier for the impedance measurements. Human epithelial stem cells (YF 29) were grown on the device surface. Cell migration speeds of 300 nm min−1 following a “scratch” wound closure assay could be established. Using a commercial software developed for geophysical prospecting, we could generate impedance tomography images at 10 kHz revealing cell migration, increase of epithelial thickness and changes in tissue resistivity over a time course of several days.

Graphical abstract: Two-dimensional impedance imaging of cell migration and epithelial stratification

Supplementary files

Article information

Article type
Paper
Submitted
15 Mar 2006
Accepted
29 Jun 2006
First published
03 Aug 2006

Lab Chip, 2006,6, 1155-1162

Two-dimensional impedance imaging of cell migration and epithelial stratification

P. Linderholm, T. Braschler, J. Vannod, Y. Barrandon, M. Brouard and P. Renaud, Lab Chip, 2006, 6, 1155 DOI: 10.1039/B603856E

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