Issue 5, 2006
From the journal:

Soft Matter

DNA polymerization on the inner surface of a giant liposome for synthesizing an artificial cell model

Koh-ichiroh Shohdaa  and  Tadashi Sugawara*a  

* Corresponding authors

a Department of Basic Science, Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8902, Japan
E-mail: suga@pentacle.c.u-tokyo.ac.jp
Fax: +81 3 5454 6997
Tel: +81 3 5454 6742

Abstract

We have designed a new artificial cell model consisting of a giant liposome, an enzyme, and DNA conjugated with a cholesterol tag by a poly(ethylene glycol) spacer to characterize the model system. The cholesterol tag of the conjugated molecule was anchored to the inner surface of the giant liposome and the single-stranded DNA unit hybridized with a 100-mer template DNA that was added to the water pool inside the liposome. We found that the DNA unit acted as a primer, DNA polymerization proceeded on the inner surface of the liposome. This reaction was a key step of our cell model. Production of a full-length strand was proved by selective cleavage of the polymerized DNA by a restriction enzyme.

Graphical abstract: DNA polymerization on the inner surface of a giant liposome for synthesizing an artificial cell model

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Article information

DOI
https://doi.org/10.1039/B516834A
Article type
Paper
Submitted
29 Nov 2005
Accepted
01 Mar 2006
First published
24 Mar 2006

Soft Matter, 2006,2, 402-408

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DNA polymerization on the inner surface of a giant liposome for synthesizing an artificial cell model

K. Shohda and T. Sugawara, Soft Matter, 2006, 2, 402 DOI: 10.1039/B516834A

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