Issue 8, 2002
From the journal:

Analyst

Determination of mercury(ii) by invertase enzyme inhibition coupled with batch injection analysis

Hasna Mohammadi,a   Mama El Rhazi,a   Aziz Amine,a   Ana Maria Oliveira Brettb  and  Christopher M. A. Brett*b  

* Corresponding authors

a Laboratoires des Analyses Chimiques et des Biocapteurs, Faculté des Sciences et Techniques, B.P. 146, Mohammedia, Morocco

b Departamento de Química, Universidade de Coimbra, 3004–535 Coimbra, Portugal
E-mail: brett@ci.uc.pt

Abstract

The determination of mercury(II) ions at the trace level by inhibition of the invertase enzyme-catalysed hydrolysis of sucrose into glucose and fructose coupled to electrochemical batch injection analysis was investigated using two approaches. In the first, the glucose produced was detected by injection of 100 μl samples into the batch injection cell containing a platinum electrode modified by immobilised glucose oxidase. In the second, the glucose and fructose present in injected samples were oxidised directly at a copper-modified glassy carbon electrode. The experimental parameters were optimised and the degree of enzyme inhibition by mercury(II) ions under both conditions was measured. Mercury concentrations in the ng ml−1 range were determined by these two techniques with low sample and reagent consumption. Comparison is made between the two methods and perspectives as a screening test for field application are indicated.

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Article information

DOI
https://doi.org/10.1039/B202887E
Article type
Paper
Submitted
22 Mar 2002
Accepted
20 Jun 2002
First published
16 Jul 2002

Analyst, 2002,127, 1088-1093

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Determination of mercury(II) by invertase enzyme inhibition coupled with batch injection analysis

H. Mohammadi, M. El Rhazi, A. Amine, A. M. O. Brett and C. M. A. Brett, Analyst, 2002, 127, 1088 DOI: 10.1039/B202887E

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