Applications of ELISA, Western blotting and immunoaffinity concentration for survey of ginsenosides in crude drugs of Panax species and traditional Chinese herbal medicines

Noriko Fukuda; Hiroyuki Tanaka; Yukihiro Shoyama

doi:10.1039/B002932G

You do not have JavaScript enabled. Please enable JavaScript to access the full features of the site or access our non-JavaScript page.

Applications of ELISA, Western blotting and immunoaffinity concentration for survey of ginsenosides in crude drugs of Panax species and traditional Chinese herbal medicines

Noriko Fukuda,^a Hiroyuki Tanaka^a and Yukihiro Shoyama*^a

Author affiliations

* Corresponding authors

^a Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, Japan.
E-mail: shoyama@shoyaku.phar.kyushu-u.ac.jp;
Fax: +92 642 6580
Tel: +92 642 6580

Abstract

A combination of ELISA, Western blotting and immunoaffinity concentration using an anti-ginsenoside Rb1 monoclonal antibody was applied for qualitative and quantitative surveys of ginsenoside Rb1 and related ginsenosides in roots and traditional Chinese herbal medicines. To improve the low correlation between ELISA and HPLC analysis, the crude extract of roots was immunoaffinity concentrated to make evident the effect of malonyl ginsenoside Rb1. Immunoaffinity column chromatography also concentrated an unknown ginsenoside which had the same cross-reaction with ginsenoside Rb1.

Download options Please wait...

Article information

DOI: https://doi.org/10.1039/B002932G
Article type: Paper
Submitted: 12 Apr 2000
Accepted: 22 Jun 2000
First published: 25 Jul 2000

Download Citation

Analyst, 2000,125, 1425-1429

Permissions

Request permissions

Applications of ELISA, Western blotting and immunoaffinity concentration for survey of ginsenosides in crude drugs of Panax species and traditional Chinese herbal medicines

N. Fukuda, H. Tanaka and Y. Shoyama, Analyst, 2000, 125, 1425 DOI: 10.1039/B002932G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Social activity

Fetching data from CrossRef.
This may take some time to load.

Analyst

Applications of ELISA, Western blotting and immunoaffinity concentration for survey of ginsenosides in crude drugs of Panax species and traditional Chinese herbal medicines

Abstract

Article information

Download Citation

Permissions

Applications of ELISA, Western blotting and immunoaffinity concentration for survey of ginsenosides in crude drugs of Panax species and traditional Chinese herbal medicines

Social activity

Search articles by author

Spotlight

Advertisements