Issue 4, 1993
From the journal:

Analyst

Development of an antibody-based amperometric biosensor to study the reaction of 7-hydroxycoumarin with its specific antibody

Eithne Dempsey,   Ciara O'Sullivan,   Malcolm R. Smyth,   Denise Egan,   Richard O'Kennedy  and  Joseph Wang  

Abstract

An amperometric biosensor, based on an anti-7-hydroxycoumarin (7-OH-coumarin) antibody immobilized at the surface of a glassy carbon electrode, and contained behind a cellulose dialysis membrane, is described. The electrochemical behaviour of this metabolite at the bare glassy carbon electrode was found to be will defined by using differential-pulse voltammetry, and a 90% decrease in peak height was observed on binding of the antibody to the antigen, which occurs at the electroactive site of 7-OH-coumarin. The kinetics of the antibody–antigen reaction was investigated and a first-order reaction was observed with k= 0.0329 mA min–1. This system provides a novel method for studying antibody specificity and the kinetics of such antibody–antigen interactions.

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Article information

DOI
https://doi.org/10.1039/AN9931800411
Article type
Paper

Analyst, 1993,118, 411-413

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Development of an antibody-based amperometric biosensor to study the reaction of 7-hydroxycoumarin with its specific antibody

E. Dempsey, C. O'Sullivan, M. R. Smyth, D. Egan, R. O'Kennedy and J. Wang, Analyst, 1993, 118, 411 DOI: 10.1039/AN9931800411

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