Issue 42, 2020

An enzyme-triggered turn-on fluorescent probe based on carboxylate-induced detachment of a fluorescence quencher

Abstract

We developed a new class of turn-on fluorescent probes for an esterase. After the esterase-mediated hydrolysis produced carboxylate (as a fluorescence activator), the fluorescence intensity was markedly increased through the detachment of a quencher moiety from the quenched Cy5 fluorophore. Because the probes based on this new activator-induced quencher-detachment (AiQd) adopt a non-immolative linker between the cleavable site and the fluorophore, the rate of the enzymatic reaction is greatly improved, without the generation of any by-products.

Graphical abstract: An enzyme-triggered turn-on fluorescent probe based on carboxylate-induced detachment of a fluorescence quencher

Supplementary files

Article information

Article type
Communication
Submitted
30 Apr 2020
Accepted
17 Aug 2020
First published
18 Aug 2020

Org. Biomol. Chem., 2020,18, 8620-8624

An enzyme-triggered turn-on fluorescent probe based on carboxylate-induced detachment of a fluorescence quencher

M. Oe, K. Miki and K. Ohe, Org. Biomol. Chem., 2020, 18, 8620 DOI: 10.1039/D0OB00899K

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