Issue 25, 2020

One-pot synthesis of heterodimeric agonists that activate the canonical Wnt signaling pathway

Abstract

Fragment antigen-binding domains (Fabs) from anti-Frizzled and anti-LRP6 monoclonal antibodies were conjugated using SpyTag–SpyCatcher chemistry via a one-pot reaction. The resulting synthetic heterodimeric agonist outperformed the natural ligand, Wnt-3a, in activating canonical Wnt signaling in mammalian cells. This approach should be broadly applicable to activate receptor-mediated cellular signaling.

Graphical abstract: One-pot synthesis of heterodimeric agonists that activate the canonical Wnt signaling pathway

Supplementary files

Article information

Article type
Communication
Submitted
04 Feb 2020
Accepted
24 Feb 2020
First published
02 Mar 2020

Chem. Commun., 2020,56, 3685-3688

Author version available

One-pot synthesis of heterodimeric agonists that activate the canonical Wnt signaling pathway

A. Mukherjee, M. E. Stathos, C. Varner, A. Arsiwala, S. Frey, Y. Hu, D. M. Smalley, D. V. Schaffer and R. S. Kane, Chem. Commun., 2020, 56, 3685 DOI: 10.1039/D0CC00920B

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