Issue 82, 2019
From the journal:

Chemical Communications

A new chemical approach for proximity labelling of chromatin-associated RNAs and proteins with visible light irradiation†

Lan Li,‡a   Jiying Liang,‡a   Hao Luo,a   K. Ming Tam, ORCID logo a   Edmund C. M. Tse ORCID logo *a  and  Ying Li ORCID logo *a  

* Corresponding authors

a Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, SAR, Hong Kong
E-mail: ecmtse@hku.hk, yingli0e@hku.hk

Abstract

A new nucleus-localized singlet oxygen generator was designed and synthesized. Its capability to label nucleus-localized biomolecules through spatially-restricted oxidation under visible light was validated using fluorescence confocal microscopy. Western blot and RT-qPCR were performed to verify the desirable spatial resolution through enriching chromatin-associated RNAs and proteins.

Graphical abstract: A new chemical approach for proximity labelling of chromatin-associated RNAs and proteins with visible light irradiation

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Article information

DOI
https://doi.org/10.1039/C9CC06251C
Article type
Communication
Submitted
12 Aug 2019
Accepted
17 Sep 2019
First published
18 Sep 2019

Chem. Commun., 2019,55, 12340-12343

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A new chemical approach for proximity labelling of chromatin-associated RNAs and proteins with visible light irradiation

L. Li, J. Liang, H. Luo, K. M. Tam, E. C. M. Tse and Y. Li, Chem. Commun., 2019, 55, 12340 DOI: 10.1039/C9CC06251C

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