Issue 51, 2019

Aptamer-based regulation of transcription circuits

Abstract

We propose synthetic DNA/RNA transcription circuits based on specific aptamer recognition. By mimicking transcription factor regulation, combined with specific enzyme/DNA aptamer binding, multiple biomolecules including DNA, RNA, polymerase, restriction enzymes and methylase were used as regulators. In addition, multi-level cascading networks and methylation-switch circuits were also established. This regulation strategy has the potential to expand the toolkit of in vitro synthetic biology.

Graphical abstract: Aptamer-based regulation of transcription circuits

Supplementary files

Article information

Article type
Communication
Submitted
23 Apr 2019
Accepted
30 May 2019
First published
31 May 2019

Chem. Commun., 2019,55, 7378-7381

Aptamer-based regulation of transcription circuits

L. Pan, Y. Hu, T. Ding, C. Xie, Z. Wang, Z. Chen, J. Yang and C. Zhang, Chem. Commun., 2019, 55, 7378 DOI: 10.1039/C9CC03141C

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