Issue 12, 2015
From the journal:

Analytical Methods

Parallelized label-free detection of protein interactions using a hyper-spectral imaging system

H. Yoshikawa,*a   M. Murahashi,a   M. Saito,a   S. Jiang,a   M. Igab  and  E. Tamiyaa  

* Corresponding authors

a Department of Applied Physics, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, Japan 565-0871
E-mail: yosikawa@ap.eng.osaka-u.ac.jp

b Innovation Head Quarters, Yokogawa Electric Corporation, Musashino, Tokyo, Japan 180-8750

Abstract

We describe parallelized label-free detection of protein interactions using a hyper-spectral imaging system. Multi-array protein chips were fabricated by immobilizing antibodies on Au-capped nanopillar polymer films, which showed an optical absorption band due to the localised surface plasmon resonance (LSPR) in the absorption spectrum. Parallelized detection of multiple antigen–antibody interactions was demonstrated by processing hyper-spectral images of the multi-array chip before and after the application of antigens. Human immunoglobulin A (IgA) and human C-reactive protein (CRP) were specifically detected, and the signal corresponding to these protein concentrations was obtained, indicating that this technique is promising for use in enhancing the performance of multiple protein assays.

Graphical abstract: Parallelized label-free detection of protein interactions using a hyper-spectral imaging system

About
Cited by
Related
Download options Please wait...

Article information

DOI
https://doi.org/10.1039/C5AY00738K
Article type
Paper
Submitted
19 Mar 2015
Accepted
12 May 2015
First published
12 May 2015

Anal. Methods, 2015,7, 5157-5161

Author version available

Download author version (PDF)

Permissions

Request permissions

Parallelized label-free detection of protein interactions using a hyper-spectral imaging system

H. Yoshikawa, M. Murahashi, M. Saito, S. Jiang, M. Iga and E. Tamiya, Anal. Methods, 2015, 7, 5157 DOI: 10.1039/C5AY00738K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements