Issue 22, 2013

Active liquid degassing in microfluidic systems

Abstract

We present a method for efficient air bubble removal in microfluidic applications. Air bubbles are extracted from a liquid chamber into a vacuum chamber through a semipermeable membrane, consisting of PDMS coated with amorphous Teflon® AF 1600. Whereas air is efficiently extracted through the membrane, water loss is greatly reduced by the Teflon even at elevated temperatures. We present the water loss and permeability change with the amount of added Teflon AF to the membrane. Also, we demonstrate bubble-free, multiplex DNA amplification using PCR in a PDMS microfluidic device.

Graphical abstract: Active liquid degassing in microfluidic systems

Article information

Article type
Paper
Submitted
27 Jun 2013
Accepted
21 Aug 2013
First published
21 Aug 2013

Lab Chip, 2013,13, 4366-4373

Active liquid degassing in microfluidic systems

J. M. Karlsson, M. Gazin, S. Laakso, T. Haraldsson, S. Malhotra-Kumar, M. Mäki, H. Goossens and W. van der Wijngaart, Lab Chip, 2013, 13, 4366 DOI: 10.1039/C3LC50778E

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