Issue 11, 2013

Microfluidic LC device with orthogonal sample extraction for on-chip MALDI-MS detection

Abstract

A microfluidic device that enables on-chip matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS) detection for liquid chromatography (LC) separations is described. The device comprises an array of functional elements to carry out LC separations, integrates a novel microchip-MS interface to facilitate the orthogonal transposition of the microfluidic LC channel into an array of reservoirs, and enables sensitive MALDI-MS detection directly from the chip. Essentially, the device provides a snapshot MALDI-MS map of the content of the separation channel present on the chip. The detection of proteins with biomarker potential from MCF10A breast epithelial cell extracts, and detection limits in the low fmol range, are demonstrated. In addition, the design of the novel LC-MALDI-MS chip entices the promotion of a new concept for performing sample separations within the limited time-frame that accompanies the dead-volume of a separation channel.

Graphical abstract: Microfluidic LC device with orthogonal sample extraction for on-chip MALDI-MS detection

Supplementary files

Article information

Article type
Paper
Submitted
10 Feb 2013
Accepted
20 Mar 2013
First published
20 Mar 2013
This article is Open Access
Creative Commons BY-NC license

Lab Chip, 2013,13, 2055-2065

Microfluidic LC device with orthogonal sample extraction for on-chip MALDI-MS detection

I. M. Lazar and J. L. Kabulski, Lab Chip, 2013, 13, 2055 DOI: 10.1039/C3LC50190F

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements