Issue 6, 2010

Surface-patterned electrode bioreactor for electrical stimulation

Abstract

We present a microscale cell culture system with an interdigitated microarray of excimer-laser-ablated indium tin oxide electrodes for electrical stimulation of cultured cells. The system has been characterized in a range of geometeries and stimulation regimes via electrochemical impedance spectroscopy and used to culture primary cardiomyocytes and human adipose derived stem cells. Over 6 days of culture with electrical stimulation (2 ms duration, 1 Hz, 180 μm wide electrodes with 200 μm spacing), both cell types exhibited enhanced proliferation, elongation and alignment, and adipose derived stem cells exhibited higher numbers of Connexin-43-composed gap junctions.

Graphical abstract: Surface-patterned electrode bioreactor for electrical stimulation

Article information

Article type
Paper
Submitted
03 Sep 2009
Accepted
27 Nov 2009
First published
05 Jan 2010

Lab Chip, 2010,10, 692-700

Surface-patterned electrode bioreactor for electrical stimulation

N. Tandon, A. Marsano, R. Maidhof, K. Numata, C. Montouri-Sorrentino, C. Cannizzaro, J. Voldman and G. Vunjak-Novakovic, Lab Chip, 2010, 10, 692 DOI: 10.1039/B917743D

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