Issue 13, 2009
From the journal:

Lab on a Chip

Resonant Rayleigh light scattering response of individual Au nanoparticles to antigen–antibody interaction†

Cuong Caoa  and  Sang Jun Sim*a  

* Corresponding authors

a Nano-optics and Biomolecular Engineering National Laboratory, Department of Chemical Engineering, Sungkyunkwan University, Suwon, Korea
E-mail: simsj@skku.edu
Fax: +82-31-290-7272
Tel: +82-31-290-7341

Abstract

A proof-of-concept study was reported on analysis of antigen–antibody recognition based on resonant Rayleigh scattering response of single Au nanoparticles in an imaging chamber. As benefited by a traditional dark-field microscope and a spectrograph, individual Au nanoparticles (30 nm) were observed with high signal-to-noise ratio and they were effectively utilized to monitor changes in refractive index induced by specific binding of the adsorbates. Using PSA antigen as a model, a LSPRλmax shift of about 2.85 nm was recorded for a molecular binding corresponding to 0.1 pg ml−1 of the protein biomarker. This result successfully demonstrates a non-labeling detection system for proteins as well as thousands of different chemical or biological species, and it possesses a great potential as a sensitive, on-chip and multiplexing detection.

Graphical abstract: Resonant Rayleigh light scattering response of individual Au nanoparticles to antigen–antibody interaction

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Article information

DOI
https://doi.org/10.1039/B901327J
Article type
Communication
Submitted
13 Feb 2009
Accepted
14 May 2009
First published
20 May 2009

Lab Chip, 2009,9, 1836-1839

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Resonant Rayleigh light scattering response of individual Au nanoparticles to antigen–antibody interaction

C. Cao and S. J. Sim, Lab Chip, 2009, 9, 1836 DOI: 10.1039/B901327J

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