Issue 9, 2009
From the journal:

Analyst

Effects of snap-freezing and near-infrared laser illumination on porcine prostate tissue as measured by Raman spectroscopy†

Stefan Candefjord,*ab   Kerstin Ramserab  and  Olof A. Lindahlab  

* Corresponding authors

a Luleå University of Technology, Department of Computer Science and Electrical Engineering, 971 87 Luleå, Sweden
E-mail: stefan.candefjord@ltu.se
Tel: +46 920 49 23 86

b , Centre for Biomedical Engineering and Physics at Luleå University of Technology

Abstract

Most Raman spectroscopic studies on tissue are performed in vitro. To assure that the results are applicable to in vivo examinations, preparation protocols and measurement procedures of tissue for in vitro studies should preserve tissue characteristics close to the native state. This study had two aims. The first was to elucidate if photoinduced effects arise during 5 minutes' continuous illumination of tissue with an 830 nm laser at an irradiance of ∼3 × 1010 W/m2. The second was to investigate the effects of snap-freezing of porcine prostate tissue in liquid nitrogen and subsequent storage at −80 °C, by means of multivariate analysis. 830 nm laser illumination of the specified irradiance did not affect the Raman spectra. A decrease of the spectral background was observed, likely due to photobleaching of tissue fluorophores. Snap-freezing and subsequent storage at −80 °C gave rise to subtle but significant alterations in Raman spectra, most likely related to changes in the protein conformations.

Graphical abstract: Effects of snap-freezing and near-infrared laser illumination on porcine prostate tissue as measured by Raman spectroscopy

About
Cited by
Related
Download options Please wait...

Article information

DOI
https://doi.org/10.1039/B820931F
Article type
Paper
Submitted
01 Dec 2008
Accepted
25 Jun 2009
First published
07 Jul 2009

Analyst, 2009,134, 1815-1821

Permissions

Request permissions

Effects of snap-freezing and near-infrared laser illumination on porcine prostate tissue as measured by Raman spectroscopy

S. Candefjord, K. Ramser and O. A. Lindahl, Analyst, 2009, 134, 1815 DOI: 10.1039/B820931F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements