Issue 8, 2002
From the journal:

Journal of Analytical Atomic Spectrometry

Simultaneous determination of proteins using an element-tagged immunoassay coupled with ICP-MS detection

Zoë A. Quinn,*a   Vladimir I. Baranov,a   Scott D. Tannera  and  Jeff L. Wranab  

* Corresponding authors

a MDS SCIEX, 71 Four Valley Drive, Concord, Ontario, Canada

b Program in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital and Department of Medical Genetics and Microbiology, University of Toronto, Ontario, Canada

Abstract

We report a novel method that suggests that multiple proteins can be quantified simultaneously in complex biological samples. The use of distinguishable element-tagged antibodies allows discriminant detection with an Inductively Coupled Plasma Mass Spectrometer (ICP-MS), providing a sensitive and accurate means of determining the concentrations of specific proteins in complex mixtures. It is demonstrated that this method can be used to detect the levels of at least two target proteins at the same time, yielding a linear response to both proteins in a concentration range of 2–100 ng mL−1 using a sample size of 0.5 mL.

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Article information

DOI
https://doi.org/10.1039/B202306G
Article type
Paper
Submitted
05 Mar 2002
Accepted
12 Jun 2002
First published
02 Jul 2002

J. Anal. At. Spectrom., 2002,17, 892-896

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Simultaneous determination of proteins using an element-tagged immunoassay coupled with ICP-MS detection

Z. A. Quinn, V. I. Baranov, S. D. Tanner and J. L. Wrana, J. Anal. At. Spectrom., 2002, 17, 892 DOI: 10.1039/B202306G

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