Issue 9, 2001
From the journal:

Journal of the Chemical Society, Perkin Transactions 2

DNA binding studies of cationic lanthanide complexes bearing a phenanthridinium group

Gabriella Bobba,a   Suzanna D. Kean,a   David Parker,*a   Andrew Beebya  and  Gail Bakera  

* Corresponding authors

a Department of Chemistry, University of Durham, South Road, Durham, UK

Abstract

The binding of tetracationic Λ- and Δ-europium complexes of tetraamide and triamide ligands incorporating an N-methylphenanthridinium group to calf-thymus DNA, poly(dGdC) and super-coiled plasmid DNA has been monitored by absorption, luminescence and CD difference spectroscopy. With the tetraamide complexes, preferential binding to (CG) base-pairs was signalled by pronounced hypochromism, a small red shift and substantial fluorescence quenching with a limiting base-pair complex ratio of 2 : 1, consistent with an intercalative interaction and charge neutralisation. For the triamide Eu complex, a lower base-pair : complex ratio was found and changes in the form and polarisation of the Eu emission spectrum were consistent with coordination of a nucleobase phosphate group to the hydrated Eu centre.

Graphical abstract: DNA binding studies of cationic lanthanide complexes bearing a phenanthridinium group

About
Cited by
Related
Download options Please wait...

Article information

DOI
https://doi.org/10.1039/B104795G
Article type
Paper
Submitted
31 May 2001
Accepted
12 Jul 2001
First published
16 Aug 2001

J. Chem. Soc., Perkin Trans. 2, 2001, 1738-1741

Permissions

Request permissions

DNA binding studies of cationic lanthanide complexes bearing a phenanthridinium group

G. Bobba, S. D. Kean, D. Parker, A. Beeby and G. Baker, J. Chem. Soc., Perkin Trans. 2, 2001, 1738 DOI: 10.1039/B104795G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Spotlight

Advertisements