Issue 17, 2012

Homogeneous assay for evaluation of aptamer–protein interaction

Abstract

We introduce Amplified Luminescent Proximity Homogenous Assay (ALPHA) to assess the KD value of aptamer–protein complexes as demonstrated through the study of apple stem pitting virus coat protein-specific aptamers. This method can be used as a simple, cost-effective method for screening aptamer–target protein interactions during aptamer selection.

Graphical abstract: Homogeneous assay for evaluation of aptamer–protein interaction

Supplementary files

Article information

Article type
Communication
Submitted
28 Mar 2012
Accepted
04 Jul 2012
First published
06 Jul 2012

Analyst, 2012,137, 3929-3931

Homogeneous assay for evaluation of aptamer–protein interaction

G. Lautner, Z. Balogh, A. Gyurkovics, R. E. Gyurcsányi and T. Mészáros, Analyst, 2012, 137, 3929 DOI: 10.1039/C2AN35419E

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