Issue 0, 1973
From the journal:

Journal of the Chemical Society, Perkin Transactions 1

Preparation of a water-insoluble trans-2,3-cyclic carbonate derivative of macroporous cellulose and its use as a matrix for enzyme immobilisation

John F. Kennedy,   S. Alan Barker  and  Alan Rosevear  

Abstract

The degree of substitution of macroporous cellulose withtrans-2,3-cyclic carbonate groups has been controlled by moderating the reaction with water. Exercise of this control enabled the preparation of a matrix with physical and chemical properties which facilitated the covalent binding of chymotrypsin A in such a way that the activity of the insoluble enzyme was appreciable towards a high molecular weight substrate (casein) as well as towards a low molecular weight substrate (tyrosine ethyl ester). Under the optimum conditions of preparation the bound protein had a relative activity towards casein of 26% and towards the ester of 65% of the activity of the free enzyme.

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Article information

DOI
https://doi.org/10.1039/P19730002293
Article type
Paper

J. Chem. Soc., Perkin Trans. 1, 1973, 2293-2299

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Preparation of a water-insoluble trans-2,3-cyclic carbonate derivative of macroporous cellulose and its use as a matrix for enzyme immobilisation

J. F. Kennedy, S. A. Barker and A. Rosevear, J. Chem. Soc., Perkin Trans. 1, 1973, 2293 DOI: 10.1039/P19730002293

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