Issue 54, 2016
From the journal:

Chemical Communications

Assessing the potential of photosensitizing flavoproteins as tags for correlative microscopy†

Alberto Rodríguez-Pulido,a   Aitziber L. Cortajarena,ab   Joaquim Torra,c   Rubén Ruiz-González,c   Santi Nonell ORCID logo c  and  Cristina Flors*a  

* Corresponding authors

a Madrid Institute for Advanced Studies in Nanoscience (IMDEA Nanoscience) and Nanobiotechnology Unit Associated to the National Center for Biotechnology (CSIC), 28049 Madrid, Spain
E-mail: cristina.flors@imdea.org

b CIC biomaGUNE, Paseo de Miramón 182, E-20009 Donostia-San Sebastian, Spain

c Institut Químic de Sarrià, Universitat Ramon Llull, 08017 Barcelona, Spain

Abstract

Photosensitizing flavoproteins have great potential as tags for correlative light and electron microscopy (CLEM). We examine the photostability of miniSOG mutants and their ability to photo-oxidize diaminobenzidine, both key aspects for CLEM. Our experiments reveal a complex relation between these parameters and the production of different reactive oxygen species.

Graphical abstract: Assessing the potential of photosensitizing flavoproteins as tags for correlative microscopy

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Article information

DOI
https://doi.org/10.1039/C6CC03119F
Article type
Communication
Submitted
14 Apr 2016
Accepted
07 Jun 2016
First published
07 Jun 2016
This article is Open Access
Creative Commons BY license

Chem. Commun., 2016,52, 8405-8408

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Assessing the potential of photosensitizing flavoproteins as tags for correlative microscopy

A. Rodríguez-Pulido, A. L. Cortajarena, J. Torra, R. Ruiz-González, S. Nonell and C. Flors, Chem. Commun., 2016, 52, 8405 DOI: 10.1039/C6CC03119F

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