A novel label-free cascade amplification strategy based on dumbbell probe-mediated rolling circle amplification-responsive G-quadruplex formation for highly sensitive and selective detection of NAD+ or ATP

Qingwang Xue; Lei Wang; Wei Jiang

doi:10.1039/C3CC39064K

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A novel label-free cascade amplification strategy based on dumbbell probe-mediated rolling circle amplification-responsive G-quadruplex formation for highly sensitive and selective detection of NAD⁺ or ATP†

Qingwang Xue,^a Lei Wang*^b and Wei Jiang*^a

Author affiliations

* Corresponding authors

^a Key Laboratory for Colloid and Interface Chemistry of Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, Jinan, P. R. China
E-mail: wjiang@sdu.edu.cn
Fax: +86 531 88564464
Tel: +86 531 88363888

^b School of Pharmacy, Shandong University, Jinan 250012, P. R. China
E-mail: wangl-sdu@sdu.edu.cn
Tel: +86 531 88363888

Abstract

A novel label-free fluorescent sensing scheme for sensitive and selective detection of NAD⁺ and ATP has been developed based on dumbbell probe-mediated rolling circle amplification (D-RCA)-responsive G-quadruplex formation. This approach can detect 0.5 pM for ATP and 100 fM for NAD⁺, much lower than those of previously reported biosensors, and exhibits high discrimination ability.

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Article information

DOI: https://doi.org/10.1039/C3CC39064K
Article type: Communication
Submitted: 19 Dec 2012
Accepted: 06 Feb 2013
First published: 07 Feb 2013

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Chem. Commun., 2013,49, 2640-2642

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A novel label-free cascade amplification strategy based on dumbbell probe-mediated rolling circle amplification-responsive G-quadruplex formation for highly sensitive and selective detection of NAD⁺ or ATP

Q. Xue, L. Wang and W. Jiang, Chem. Commun., 2013, 49, 2640 DOI: 10.1039/C3CC39064K

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Chemical Communications