Issue 6, 2013
From the journal:

Analytical Methods

Boronate affinity solid-phase extraction based on functionalized magnesia–zirconia composite for enrichment of nucleosides in human urine

Hai-bo He,*a   Yan-ran Sun,a   Bin Li,a   Qiong-wei Yu,b   Tian-lin Wanga  and  Yu-qi Feng*b  

* Corresponding authors

a Department of chemistry, Shanghai University, Shanghai 200444, P. R. China
Web: hbhe2006@shu.edu.cn
Fax: +86-21-66134594
Tel: 86-2166132930

b Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, P. R. China
Web: yqfeng@whu.edu.cn
Fax: +86-27-68755595
Tel: +86-27-68755595

Abstract

A novel boronic acid functionalized magnesia–zirconia (ZM) based material was facile synthesized by Lewis acid–base chemisorption. The elemental analysis, inductively coupled plasma atomic emission spectrometer (ICP-AES) and Fourier transform infrared spectrometer (FT-IR) have proven the successful attachment of 3-aminophenylboronic acid derivative onto ZM surface. In the present work, the affinity of the resulting material towards cis-diols binding was evaluated by using it as the sorbent for solid-phase extraction of 4 nucleosides (namely cytidine, uridine, guanine and adenosine). The results show that such boronic acid modified magnesia–zirconia composite microparticles were excellent for capturing nucleosides in the system with a wide range of pH and high salt concentration, which could be effectively released using TFA–ACN–H2O (5 : 50 : 45, v/v/v) solution as elution. The 4 nucleosides spiked to untreated urine sample (i.e., no diluting or pH adjustment) were prominently enriched with recoveries in the range of 86–109% and LOD values in the range of 0.003–0.010 μg mL−1. The constructed calibration curves range from 0.05 to 2.0 μg mL−1 showing good linearity correlation coefficient (R ≥ 0.9961) and precision (RSD < 9%). The proposed material would allow for an alternative extraction strategy for identification and quantitative analysis of cis-diols structure compounds in biological samples.

Graphical abstract: Boronate affinity solid-phase extraction based on functionalized magnesia–zirconia composite for enrichment of nucleosides in human urine

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Article information

DOI
https://doi.org/10.1039/C2AY26420J
Article type
Paper
Submitted
19 Nov 2012
Accepted
24 Dec 2012
First published
08 Jan 2013

Anal. Methods, 2013,5, 1435-1441

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Boronate affinity solid-phase extraction based on functionalized magnesia–zirconia composite for enrichment of nucleosides in human urine

H. He, Y. Sun, B. Li, Q. Yu, T. Wang and Y. Feng, Anal. Methods, 2013, 5, 1435 DOI: 10.1039/C2AY26420J

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