Issue 14, 2011
From the journal:

Lab on a Chip

Biocompatible multi-address 3D cell assembly in microfluidic devices using spatially programmable gel formation†

Yi Cheng,a   Xiaolong Luo,b   Chen-Yu Tsao,b   Hsuan-Chen Wu,bc   Jordan Betz,ac   Gregory F. Payne,bc   William E. Bentleybc  and  Gary W. Rubloff*ad  

* Corresponding authors

a Institute for Systems Research (ISR), University of Maryland, College Park, MD, USA
E-mail: rubloff@umd.edu
Fax: +1 301 314-9920
Tel: +1 301 405-2949

b Institute for Bioscience and Biotechnology Research (IBBR), University of Maryland, College Park, MD, USA

c Fischell Department of Bioengineering, University of Maryland, College Park, MD, USA

d Department of Materials Science and Engineering, University of Maryland, College Park, MD, USA

Abstract

Programmable 3D cell assembly under physiological pH conditions is achieved using electrodeposited stimuli-responsive alginate gels in a microfluidic device, with parallel sidewall electrodes enabling direct observation of the cell assembly. Electrically triggered assembly and subsequent viability of mammalian cells is demonstrated, along with spatially programmable, multi-address assembly of different strains of E. colicells. Our approach enables in vitro study of dynamic cellular and inter-cellular processes, from cell growth and stimulus/response to inter-colony and inter-species signaling.

Graphical abstract: Biocompatible multi-address 3D cell assembly in microfluidic devices using spatially programmable gel formation

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Article information

DOI
https://doi.org/10.1039/C1LC20306A
Article type
Communication
Submitted
11 Apr 2011
Accepted
12 May 2011
First published
01 Jun 2011

Lab Chip, 2011,11, 2316-2318

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Biocompatible multi-address 3D cell assembly in microfluidic devices using spatially programmable gel formation

Y. Cheng, X. Luo, C. Tsao, H. Wu, J. Betz, G. F. Payne, W. E. Bentley and G. W. Rubloff, Lab Chip, 2011, 11, 2316 DOI: 10.1039/C1LC20306A

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