Issue 18, 2011
From the journal:

Analyst

Comprehensive native glycan profiling with isomer separation and quantitation for the discovery of cancer biomarkers†

Serenus Hua,a   Hyun Joo An,‡*b   Sureyya Ozcan,a   Grace S. Ro,a   Stephanie Soares,c   Ralph DeVere-Whitec  and  Carlito B. Lebrilla‡*ad  

* Corresponding authors

a Department of Chemistry, University of California, Davis, USA
E-mail: cblebrilla@ucdavis.edu
Fax: +1 530-752-8995
Tel: +1 530-752-0504

b Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Korea
E-mail: hjan@cnu.ac.kr
Tel: +82 42-821-8547

c UC Davis Cancer Center, University of California, Davis, USA

d Department of Biochemistry and Molecular Medicine, University of California, Davis, USA

Abstract

Glycosylation is highly sensitive to the biochemical environment and has been implicated in many diseases including cancer. Glycan compositional profiling of human serum with mass spectrometry has already identified potential biomarkers for several types of cancer and diseases; however, composition alone does not fully describe glycan stereo- and regioisomeric diversity. The vast structural heterogeneity of glycans presents a formidable analytical challenge. We have developed a method to identify and quantify isomeric native glycans using nanoflow liquid chromatography (nano-LC)/mass spectrometry. A microfluidic chip packed with graphitized carbon was used to chromatographically separate the glycans. To determine the utility of this method for structure-specific biomarker discovery, we analyzed serum samples from two groups of prostate cancer patients with different prognoses. More than 300 N-glycan species (including isomeric structures) were identified, corresponding to over 100 N-glycan compositions. Statistical tests established significant differences in glycan abundances between patient groups. This method provides comprehensive, selective, and quantitative glycan profiling.

Graphical abstract: Comprehensive native glycan profiling with isomer separation and quantitation for the discovery of cancer biomarkers

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Article information

DOI
https://doi.org/10.1039/C1AN15093F
Article type
Paper
Submitted
01 Feb 2011
Accepted
24 Jun 2011
First published
21 Jul 2011

Analyst, 2011,136, 3663-3671

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Comprehensive native glycan profiling with isomer separation and quantitation for the discovery of cancer biomarkers

S. Hua, H. J. An, S. Ozcan, G. S. Ro, S. Soares, R. DeVere-White and C. B. Lebrilla, Analyst, 2011, 136, 3663 DOI: 10.1039/C1AN15093F

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