Issue 8, 2010
From the journal:

Analyst

Antibody functionalized interdigitated μ-electrode (IDμE) based impedimetric cortisol biosensor†

Sunil K. Arya,*a   Ganna Chornokur,a   Manju Venugopalb  and  Shekhar Bhansali*a  

* Corresponding authors

a BioMEMS and Microsystems Lab, Department of Electrical Engineering, University of South Florida, 4202 E. Fowler Avenue, ENB 118, Tampa, FL 33620, USA
E-mail: sunilarya333@gmail.com, bhansali@usf.edu

b Guided Therapeutics Inc., 5835 Peachtree Corners East, Suite D, Norcross, GA 30092, USA

Abstract

This paper reports on an ultrasensitive, disposable, impedimetric biosensor for cortisol detection. C-Mab (a Cortisol specific monoclonal antibody) was covalently immobilized via amide bond on the surface of the interdigitated μ-electrodes (IDμEs) functionalized with dithiobis(succinimidyl propionate) (DTSP) self-assembled monolayer (SAM). After C-Mab binding, unreacted active groups of DTSP were blocked using ethanolamine (EA) and glycine (Gly) mixture. The disposable sensors were exposed to solutions with different cortisol concentrations and a label-free electrochemical impedance (EIS) technique was used to determine the cortisol concentration. EIS results confirm that the EA-Gly/C-Mab/DTSP/IDμE based biosensor exhibited the sensitivity of 2.855 kohms M−1 and could accurately detect cortisol in the range of 1 pM to 10 nM in saliva. This work establishes the feasibility of using an impedance based biosensor as a disposable cortisol detector, capable of working with complex bodily fluids (e.g., saliva). The architecture enables the use of cortisol sensors at point-of-care.

Graphical abstract: Antibody functionalized interdigitated μ-electrode (IDμE) based impedimetric cortisol biosensor

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Article information

DOI
https://doi.org/10.1039/C0AN00242A
Article type
Paper
Submitted
16 Apr 2010
Accepted
05 Jun 2010
First published
29 Jun 2010

Analyst, 2010,135, 1941-1946

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Antibody functionalized interdigitated μ-electrode (IDμE) based impedimetric cortisol biosensor

S. K. Arya, G. Chornokur, M. Venugopal and S. Bhansali, Analyst, 2010, 135, 1941 DOI: 10.1039/C0AN00242A

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