Issue 12, 2010
From the journal:

Soft Matter

Quantum dotprobes for observation of single molecule DNA and a synthetic polyelectrolyte higher-order structure†

Ning Chen,ab   Anatoly A. Zinchenko,*ab   Yuka Yamazaki,a   Yuko Yoshikawa,c   Shizuaki Murataa  and  Kenichi Yoshikawacd  

* Corresponding authors

a Graduate School of Environmental Studies, Nagoya University, Furou-cho, Nagoya, Japan
E-mail: zinchenko@urban.env.nagoya-u.ac.jp

b Spatio-Temporal Order Project, ICORP, JST, Tokyo, Japan

c Research Organization of Science and Engineering, Ritsumeikan University, Kusatsu, Japan

d Department of Physics, Graduate School of Science, Kyoto University, Kyoto, Japan

Abstract

Semiconductor nanocrystals (quantum dots) have advantageous optical properties compared to conventional organic fluorescent dyes. At present, quantum dots are widely used for the observation of cells and tissues, labeling of short oligonucleotide sequences for the detection of hybridization, and other biological purposes. However, the possibility to observe long macromolecules with quantum dots has not yet been examined. In this report we present the results of our investigation on the labeling of long DNA molecules, as well as a long synthetic polyelectrolyte (sodium polystyrene sulfonate), with quantum dots as a first experimental protocol for the quantum dot-based single-molecule observation of long polymer chains. We examined a suitability of Qdots as fluorescent binders to monitor conformational transitions of giant DNA and polystyrene sulfonate labeled with Qdots. The advantages and drawbacks of Qdots as fluorescent probes for polymers are described.

Graphical abstract: Quantum dot probes for observation of single molecule DNA and a synthetic polyelectrolyte higher-order structure

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Supplementary files

Article information

DOI
https://doi.org/10.1039/C001686A
Article type
Paper
Submitted
29 Jan 2010
Accepted
17 Apr 2010
First published
14 May 2010

Soft Matter, 2010,6, 2834-2841

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Quantum dot probes for observation of single molecule DNA and a synthetic polyelectrolyte higher-order structure

N. Chen, A. A. Zinchenko, Y. Yamazaki, Y. Yoshikawa, S. Murata and K. Yoshikawa, Soft Matter, 2010, 6, 2834 DOI: 10.1039/C001686A

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