Issue 12, 2010

Human mammalian cell sorting using a highly integrated micro-fabricated fluorescence-activated cell sorter (μFACS)

Abstract

We demonstrate a high performance microfabricated FACS system with highly integrated microfluidics, optics, acoustics, and electronics. Single cell manipulation at a high speed is made possible by the fast response time (∼0.1 ms) of the integrated PZT actuator and the nozzle structure at the sorting junction. A Teflon AF-coated optofluidic waveguide along the microfluidic channel guides the illumination light, enabling multi-spot detection, while a novel space-time coding technology enhances the detection sensitivity of the μFACS system. The real-time control loop system is implemented using a field-programmable-gate-array (FPGA) for automated and accurate sorting. The μFACS achieves a high purification enrichment factor: up to ∼230 fold for both polystyrene microbeads and suspended human mammalian cells (K562) at a high throughput (>1000 cells s−1). The sorting mechanism is independent of cell properties such as size, density, and shape, thus the presented system can be applied to sort out any pure sub-populations. This new lab-on-a-chip FACS system, therefore, holds promise to revolutionize microfluidic cytometers to meet cost, size, and performance goals.

Graphical abstract: Human mammalian cell sorting using a highly integrated micro-fabricated fluorescence-activated cell sorter (μFACS)

Article information

Article type
Paper
Submitted
07 Jan 2010
Accepted
09 Mar 2010
First published
09 Apr 2010

Lab Chip, 2010,10, 1567-1573

Human mammalian cell sorting using a highly integrated micro-fabricated fluorescence-activated cell sorter (μFACS)

S. H. Cho, C. H. Chen, F. S. Tsai, J. M. Godin and Y. Lo, Lab Chip, 2010, 10, 1567 DOI: 10.1039/C000136H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements