Issue 8, 2005
From the journal:

Organic & Biomolecular Chemistry

Investigation of the interaction between peanut agglutinin and synthetic glycopolymeric multivalent ligands†

Moira Ambrosi,a   Neil R. Cameron,*a   Benjamin G. Davis*b  and  Snjezana Stolnikc  

* Corresponding authors

a Department of Chemistry and Interdisciplinary Research Centre in Polymer Science and Technology, University of Durham, South Road, Durham, UK
E-mail: n.r.cameron@durham.ac.uk

b Department of Chemistry, University of Oxford, Chemical Research Laboratory, Mansfield Road, Oxford, UK
E-mail: ben.davis@chem.ox.ac.uk

c School of Pharmaceutical Sciences, University of Nottingham, University Park, Nottingham, UK

Abstract

The interaction between synthetic glycoplymers bearing β-D-galactose side groups and the lectin peanut agglutinin (PNA) was investigated by UV-difference spectroscopy and isothermal titration calorimetry (ITC). UV-difference spectroscopy indicated that the polymer–lectin interaction was stronger than that between PNA and either the corresponding monomer, D-galactose or D-lactose. The thermodynamics of binding (K, ΔG, ΔH, ΔS and n) were determined from ITC data by fitting with a two-site, non-cooperative binding model. It was found that the glycopolymer displayed around a 50 times greater affinity for the lectin than the parent carbohydrate, and around 10 times greater than the monomer, on a valency-corrected basis. Binding was found to be entropically driven, and was accompanied by aggregation and precipitation of protein molecules. Furthermore, interesting differences between polymers prepared either from deacetylated monomers, or by deacetylation of pre-formed polymers, were found.

Graphical abstract: Investigation of the interaction between peanut agglutinin and synthetic glycopolymeric multivalent ligands

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Article information

DOI
https://doi.org/10.1039/B411555B
Article type
Paper
Submitted
27 Jul 2004
Accepted
21 Feb 2005
First published
07 Mar 2005

Org. Biomol. Chem., 2005,3, 1476-1480

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Investigation of the interaction between peanut agglutinin and synthetic glycopolymeric multivalent ligands

M. Ambrosi, N. R. Cameron, B. G. Davis and S. Stolnik, Org. Biomol. Chem., 2005, 3, 1476 DOI: 10.1039/B411555B

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