Issue 12, 2003
From the journal:

Analyst

An amobarbital molecularly imprinted microsphere for selective solid-phase extraction of phenobarbital from human urine and medicines and their determination by high-performance liquid chromatography

Shu-Guo Hu,a   Shan-Wei Wangb  and  Xi-Wen He*a  

* Corresponding authors

a Department of Chemistry, State Key Laboratory of Functional Polymer Materials for Adsorption and Separation, Nankai University, Tianjin 300071, PR China

b Center of Test, Nankai University, Tianjin 300071, PR China

Abstract

A 40–60 µm amobarbital molecularly imprinted microsphere, used as a solid-phase selective sorbent for extracting phenobarbital from human urine and medicines, was prepared by a suspension polymerization method. A series of binding studies was performed in order to find optimal loading, washing and eluting conditions for solid-phase extraction. Under optimal conditions, good recoveries of phenobarbital in samples were obtained. Normally, molecularly imprinted polymers, prepared in bulk, require laborious work. Significant losses occur during the procedure of grinding and washing. In this work all molecularly imprinted polymers made into microsphere could be utilized, and the cost of the template was reduced too (the price of phenobarbital is twice that of amobarbital). As the phenobarbital to be extracted was different from the template molecule amobarbital, the interference caused by template leaking could be avoided in the assay.

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Article information

DOI
https://doi.org/10.1039/B310775B
Article type
Paper
Submitted
04 Sep 2003
Accepted
22 Oct 2003
First published
13 Nov 2003

Analyst, 2003,128, 1485-1489

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An amobarbital molecularly imprinted microsphere for selective solid-phase extraction of phenobarbital from human urine and medicines and their determination by high-performance liquid chromatography

S. Hu, S. Wang and X. He, Analyst, 2003, 128, 1485 DOI: 10.1039/B310775B

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