Issue 29, 2020

Gold nanoparticle-engineered electrochemical aptamer biosensor for ultrasensitive detection of thrombin

Abstract

In order to obtain a lower detection limit in electrochemical detection, the choice of signal amplification strategy is of great importance. In this work, we describe an electrochemical sandwich aptasensor based on a signal amplification system involving two thrombin (TB) aptamers (TBA1 and TBA2), gold nanoparticles (AuNPs) as aptamer carriers, and [Ru(NH3)6]3+ for signal conversion. In the presence of the target thrombin, TBA1 and TBA2 specifically bind to TB, and the TBA1–TB–TBA2 complexes cause the formation of a sandwich structure, meaning more [Ru(NH3)6]3+ can be adsorbed on the negatively charged phosphate backbone of the aptamers, resulting in an increase in the differential pulse voltammetry (DPV) current. Under optimal conditions, the aptasensor exhibited a linear range of 1 fM to 6 pM and a limit of detection of 0.1429 fM (S/N = 3) for TB. The proposed aptasensor displayed an excellent selectivity and reproducibility. Importantly, the aptasensor was capable of detecting TB in serum samples successfully.

Graphical abstract: Gold nanoparticle-engineered electrochemical aptamer biosensor for ultrasensitive detection of thrombin

Article information

Article type
Paper
Submitted
12 Jun 2020
Accepted
03 Jul 2020
First published
03 Jul 2020

Anal. Methods, 2020,12, 3729-3733

Gold nanoparticle-engineered electrochemical aptamer biosensor for ultrasensitive detection of thrombin

Y. Chen, J. Xiang, B. Liu, Z. Chen and X. Zuo, Anal. Methods, 2020, 12, 3729 DOI: 10.1039/D0AY01163K

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