Issue 38, 2018, Issue in Progress
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RSC Advances

Dual-spectra encoded suspension array using reversed-phase microemulsion UV curing and electrostatic self-assembling

Guangxia Feng,†ab   Qinghua He,†ac   WenYue Xie,d   Yonghong He, ORCID logo *ac   Xuejing Chen,ac   Bei Wang,a   Bangrong Lua  and  Tian Guan*ab  

* Corresponding authors

a Institute of Optical Imaging and Sensing, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, People's Republic of China
E-mail: heyh@sz.tsinghua.edu.cn, guantian@sz.tsinghua.edu.cn

b School of Medicine, Tsinghua University, Beijing 100084, People's Republic of China

c Department of Physics, Tsinghua University, Beijing 100084, People's Republic of China

d Department of Clinical Laboratory, Peking University ShenZhen Hospital, China

Abstract

The rapid growth of demand for high-throughput multiplexed biochips from modern biotechnology has led to growing interest in suspension array based on multi-channel encoded microbeads. We prepare dual-spectra encoded PEGDA microbeads (DSEPM) by reversed-phase microemulsion UV curing method and layer-by-layer electrostatic self-assembly method. Excitation of the synthesized DSEPM results in two spectra, including fluorescence spectra from quantum dots and laser induced breakdown spectra from nanoparticles with specific elements. With further surface modification and bio-probes grafting, we use DSEPM to carry a series of detection experiments of biomolecules. The adsorption experiment to two types of anti-IgG in mixture sample has demonstrated the availability of DSEPM in multiplexing. Then, the contrast experiment has verified the specificity of DSEPM in detection. Finally, we carry out the concentration gradient experiment and obtain the response curve to show the performance of DSEPM in quantitative analysis. The results indicate our method provide an effective way to improve multiplexed biochips with more coding capacity, accuracy and stability.

Graphical abstract: Dual-spectra encoded suspension array using reversed-phase microemulsion UV curing and electrostatic self-assembling

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Article information

DOI
https://doi.org/10.1039/C8RA02410C
Article type
Paper
Submitted
19 Mar 2018
Accepted
16 May 2018
First published
11 Jun 2018
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2018,8, 21272-21279

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Dual-spectra encoded suspension array using reversed-phase microemulsion UV curing and electrostatic self-assembling

G. Feng, Q. He, W. Xie, Y. He, X. Chen, B. Wang, B. Lu and T. Guan, RSC Adv., 2018, 8, 21272 DOI: 10.1039/C8RA02410C

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