Issue 7, 2018, Issue in Progress
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RSC Advances

A rapid and simple UPLC-MS/MS method using collagen marker peptides for identification of porcine gelatin

Shangwei Guo,ab   Xiaobing Xu,a   Xiangshan Zhoub  and  Yaqin Huang ORCID logo *a  

* Corresponding authors

a Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China
E-mail: huangyq@mail.buct.edu.cn

b Dong'e E-jiao Co., Ltd, National Engineering Research Center for Gelatine TCM, Liaocheng 252201, China

Abstract

Gelatin, which is mainly derived from bovine and porcine sources, has been used in many foods and pharmaceutical products. To ensure the compliance of food products with halal regulations, reliable analytical methods are very much required. In this study, one unique marker peptide for porcine gelatins was selected to develop an UPLC-MS/MS multiple reaction monitoring method. The capability of this method to identify porcine materials was demonstrated by analyzing in-house-made gelatins containing different amounts of porcine gelatins and commercial gelatin products. The adulteration of porcine gelatin could be sensitively detected at a low level of 0.04%. When combined with HPLC and mass spectrometry, this method is an accurate and sensitive quantitative method to identify porcine gelatins. Thus, the strategy can be used to verify halal authenticity of gelatin.

Graphical abstract: A rapid and simple UPLC-MS/MS method using collagen marker peptides for identification of porcine gelatin

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Article information

DOI
https://doi.org/10.1039/C7RA12539A
Article type
Paper
Submitted
17 Nov 2017
Accepted
12 Jan 2018
First published
19 Jan 2018
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2018,8, 3768-3773

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A rapid and simple UPLC-MS/MS method using collagen marker peptides for identification of porcine gelatin

S. Guo, X. Xu, X. Zhou and Y. Huang, RSC Adv., 2018, 8, 3768 DOI: 10.1039/C7RA12539A

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