Colorimetric detection of Hg2+ based on inhibiting the peroxidase-like activity of DNA–Ag/Pt nanoclusters

Liang-Liang Wu; Li-Ying Wang; Zheng-Jun Xie; Feng Xue; Chi-Fang Peng

doi:10.1039/C6RA12597B

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Colorimetric detection of Hg²⁺ based on inhibiting the peroxidase-like activity of DNA–Ag/Pt nanoclusters†

Liang-Liang Wu,^a Li-Ying Wang,^a Zheng-Jun Xie,^a Feng Xue*^b and Chi-Fang Peng

*^a

Author affiliations

* Corresponding authors

^a State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, China
E-mail: pcf@jiangnan.edu.cn

^b Animal, Plant and Food Inspection Center, Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing, China
E-mail: fengxue1219@aliyun.com

Abstract

This paper reported that the peroxidase-like activity of DNA–Ag/Pt nanoclusters (NCs) can be inhibited selectively by Hg²⁺. The interaction between Hg²⁺ and the DNA–Ag/Pt NCs was discussed through characterization by TEM, DLS and XPS, etc. It was found that the integration of both Hg⁰ and Hg²⁺ to the DNA–Ag/Pt NCs resulted in the DNA–Ag/Pt NCs aggregation and the decrease of Pt²⁺ content on the DNA–Ag/Pt NCs surface, which finally impaired their catalytic activity. Based on the above mechanism, a colorimetric detection for Hg²⁺ was developed with a detection limit (LOD) of 5.0 nM and a linear range from 10 nM to 200 nM. The method was highly selective toward Hg²⁺ over other common metal ions, low-cost and simple, which facilitated the application of detecting Hg²⁺ in tap water.

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Article information

DOI: https://doi.org/10.1039/C6RA12597B
Article type: Paper
Submitted: 15 May 2016
Accepted: 28 Jul 2016
First published: 28 Jul 2016

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RSC Adv., 2016,6, 75384-75389

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Colorimetric detection of Hg²⁺ based on inhibiting the peroxidase-like activity of DNA–Ag/Pt nanoclusters

L. Wu, L. Wang, Z. Xie, F. Xue and C. Peng, RSC Adv., 2016, 6, 75384 DOI: 10.1039/C6RA12597B

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