Issue 23, 2016
From the journal:

Organic & Biomolecular Chemistry

Clamping of RNA with PNA enables targeting of microRNA†

Alice Ghidini,a   Helen Bergquist,b   Merita Murtola,ac   Tanel Punga,b   Rula Zainde  and  Roger Strömberg*a  

* Corresponding authors

a Department of Biosciences and Nutrition, Karolinska Institutet, Novum, 141 83 Huddinge, Stockholm, Sweden
E-mail: Roger.Stromberg@ki.se

b Department of Medical Biochemistry and Microbiology, Uppsala University, BMC, 75123 Uppsala, Sweden

c Department of Chemistry, University of Turku, 20014 Turku, Finland

d Department of Laboratory Medicine, Clinical Research Center, Karolinska Institutet, 141 86 Huddinge, Stockholm, Sweden

e Centre for Rare Diseases, Karolinska University Hospital, Stockholm, Sweden

Abstract

To be able to target microRNAs also at stages where these are in a double stranded or hairpin form we have studied BisPNA designed to clamp the target and give sufficient affinity to allow for strand invasion. We show that BisPNA complexes are more stable with RNA than with DNA. In addition, 24-mer BisPNA (AntimiR) constructs form complexes with a hairpin RNA that is a model of the microRNA miR-376b, suggesting that PNA-clamping may be an effective way of targeting microRNAs.

Graphical abstract: Clamping of RNA with PNA enables targeting of microRNA

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Article information

DOI
https://doi.org/10.1039/C6OB00516K
Article type
Communication
Submitted
07 Mar 2016
Accepted
12 May 2016
First published
16 May 2016

Org. Biomol. Chem., 2016,14, 5210-5213

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Clamping of RNA with PNA enables targeting of microRNA

A. Ghidini, H. Bergquist, M. Murtola, T. Punga, R. Zain and R. Strömberg, Org. Biomol. Chem., 2016, 14, 5210 DOI: 10.1039/C6OB00516K

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