Issue 28, 2016
From the journal:

Analytical Methods

A rapid quantitative 1H NMR analysis of kinsenoside and other bioactive principles from Anoectochilus formosanus

Li-Ying Hsieh,a   Hsiu-Hui Chan,a   Hsin-Yi Hung,b   Chao-Lin Kuo,c   Jin-Bin Wu,d   I-Wen Sun,a   Ping-Chung Kuo*b  and  Tian-Shung Wu*be  

* Corresponding authors

a Department of Chemistry, National Cheng Kung University, Tainan 701, Taiwan, Republic of China

b School of Pharmacy, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan, Republic of China
E-mail: z10502016@email.ncku.edu.tw, tswu@mail.ncku.edu.tw
Fax: +886-6-2747538
Tel: +886-6-2747538

c Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 401, Taiwan, Republic of China

d School of Pharmacy, China Medical University, Taichung 401, Taiwan, Republic of China

e Department of Pharmacy and Graduate Institute of Pharmaceutical Technology, Tajen University, Pingtung 90741, Taiwan, Republic of China

Abstract

Anoectochilus formosanus is a famous traditional Taiwanese folk medicine. Kinsenoside (1), one of the major active components in A. formosanus, exhibits hepatoprotective, antihyperliposis and antiflammatory activities. In this study, a proton nuclear magnetic resonance (1H NMR) method was developed for rapid quantitative analysis of several bioactive major constituents, including kinsenoside (1), 3-(R)-3-β-D-gluco-pyranosyloxy-4-hydroxybutanoic acid (2), 1-O-isopropyl-β-D-gluco-pyranoside (3), 4-(β-D-gluco-pyranosyloxy)benzyl alcohol (4) and benzoic acid (5) in plant samples and commercial products of A. formosanus. Interferences were removed by a simple Diaion HP-20 column chromatography. In the 1H NMR spectrum, the signals of compounds 1 (δ 2.88), 2 (δ 2.47), 3 (δ 1.12), 4 (δ 7.05) and 5 (δ 7.84) were selected and integrated as the target peaks, separated well from other signals in D2O. The developed 1H NMR method provides a rapid and simple quantitative method without any derivatization, pH control or addition of NMR shift reagent, and the analytical solvent system is very safe, convenient and simple.

Graphical abstract: A rapid quantitative 1H NMR analysis of kinsenoside and other bioactive principles from Anoectochilus formosanus

About
Cited by
Related
Download options Please wait...

Article information

DOI
https://doi.org/10.1039/C6AY00670A
Article type
Paper
Submitted
08 Mar 2016
Accepted
12 Jun 2016
First published
15 Jun 2016

Anal. Methods, 2016,8, 5645-5650

Permissions

Request permissions

A rapid quantitative 1H NMR analysis of kinsenoside and other bioactive principles from Anoectochilus formosanus

L. Hsieh, H. Chan, H. Hung, C. Kuo, J. Wu, I. Sun, P. Kuo and T. Wu, Anal. Methods, 2016, 8, 5645 DOI: 10.1039/C6AY00670A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements