Issue 24, 2016

Phage-displayed macrocyclic glycopeptide libraries

Abstract

In this report, we describe an efficient way to generate libraries of macrocyclic glycopeptides in one step by reacting phage-displayed libraries of peptides with dichloro-oxime derivatives. We showed that the reactions do not interfere with the ability of phage to replicate in bacteria. The reactions are site-selective for phage-displayed peptides and they do not modify any other proteins of phage. The technology described in this report will be instrumental for genetic selection of macrocyclic glycopeptides for diverse glycan-binding proteins.

Graphical abstract: Phage-displayed macrocyclic glycopeptide libraries

  • This article is part of the themed collection: New Talent

Supplementary files

Article information

Article type
Communication
Submitted
24 Dec 2015
Accepted
02 Feb 2016
First published
04 Feb 2016
This article is Open Access
Creative Commons BY license

Org. Biomol. Chem., 2016,14, 5539-5545

Phage-displayed macrocyclic glycopeptide libraries

S. Ng and R. Derda, Org. Biomol. Chem., 2016, 14, 5539 DOI: 10.1039/C5OB02646F

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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