Magnetically guided survivin-siRNA delivery and simultaneous dual-modal imaging visualization based on Fe3O4@mTiO2 nanospheres for breast cancer

Jiang Wu; Ying Liu; Wei Li; Chunyan Wang; Yanjun Li; Ying Tian; Jing Sun; Shouju Wang; Xin Wang; Yuxia Tang; Hong Zhu; Zhaogang Teng; Guangming Lu

doi:10.1039/C4TB01264J

Magnetically guided survivin-siRNA delivery and simultaneous dual-modal imaging visualization based on Fe₃O₄@mTiO₂ nanospheres for breast cancer†

Jiang Wu,‡^a Ying Liu,‡^b Wei Li,^c Chunyan Wang,^b Yanjun Li,^b Ying Tian,^b Jing Sun,^b Shouju Wang,^b Xin Wang,^b Yuxia Tang,^b Hong Zhu,^a Zhaogang Teng*^b and Guangming Lu*^b

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* Corresponding authors

^a Department of Nuclear Medicine, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, P.R. China

^b Department of Medical Imaging, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, P.R. China
E-mail: cjr.luguangming@vip.163.com, tzg@fudan.edu.cn
Fax: +86 25 8480 4659
Tel: +86 25 8086 0185

^c Department of Chemistry, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, and Laboratory of Advanced Materials, Fudan University, Shanghai 200433, P. R. China

Abstract

Small interfering RNA (siRNA) has great potential for gene therapy of breast cancer. The development of multifunctional nanocarriers possessing the targeted ability and multimodal imaging function is important to increase siRNA transfection efficiency and to visualize the delivery events. Herein, we demonstrate flavin mononucleotide (FMN) and polyethylenimine (PEI) modified Fe₃O₄@mTiO₂ nanoparticles (Fe₃O₄@mTiO₂/FMN-PEI) as novel magnetic fluorescent siRNA delivery systems. The system has magnetic targeting, magnetic resonance imaging (MRI), optical imaging, and high siRNA binding ability. Survivin-siRNA delivered by this system can effectively enter into MCF-7 cells, which can be further enhanced by exerting an external magnetic field. More importantly, the siRNA delivery events including the location, trafficking, distribution, and release, can be visualized by MRI and optical imaging. Gene silencing by survivin-siRNA can cause significant protein knockdown (96%) and considerable cell apoptosis (30%), indicating the usefulness of Fe₃O₄@mTiO₂/FMN-PEI as a siRNA delivery system.

Journal of Materials Chemistry B

Magnetically guided survivin-siRNA delivery and simultaneous dual-modal imaging visualization based on Fe₃O₄@mTiO₂ nanospheres for breast cancer†

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Magnetically guided survivin-siRNA delivery and simultaneous dual-modal imaging visualization based on Fe₃O₄@mTiO₂ nanospheres for breast cancer

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Journal of Materials Chemistry B