Issue 3, 2012
From the journal:

Chemical Science

Mouse lactate dehydrogenase X: A promising magnetic resonance reporter protein using hyperpolarized pyruvic acid derivative Y†

Tatsuya Nishihara,a   Hiroshi Nonaka,a   Tatsuya Naganuma,b   Kazuhiro Ichikawac  and  Shinsuke Sando*a  

* Corresponding authors

a INAMORI Frontier Research Center, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, Japan
E-mail: ssando@ifrc.kyushu-u.ac.jp
Fax: (+81) 92-802-6960

b Japan Redox, Ltd., 4-29-49-805 Chiyo, Hakata-ku, Fukuoka, Japan

c Innovation Center for Medical Redox Navigation, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka, Japan

Abstract

We developed a promising candidate for a hypersensitive magnetic resonance (MR) reporter protein. The reporter protein is comprised of mouse lactate dehydrogenase-X (mLDH-X) and a hyperpolarized pyruvic acid derivative, substrate Y that gives no false-positive signals by endogenous human lactate dehydrogenases (hLDHs). The X–Y pair shows high MR sensitivity and sufficient reaction kinetics, thus enabling direct detection of reporter protein X in crude biological mixtures by monitoring the 13C NMR signal of hyperpolarized product Yr.

Graphical abstract: Mouse lactate dehydrogenase X: A promising magnetic resonance reporter protein using hyperpolarized pyruvic acid derivative Y

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Supplementary files

Article information

DOI
https://doi.org/10.1039/C1SC00919B
Article type
Edge Article
Submitted
14 Nov 2011
Accepted
15 Nov 2011
First published
01 Dec 2011

Chem. Sci., 2012,3, 800-806

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Mouse lactate dehydrogenase X: A promising magnetic resonance reporter protein using hyperpolarized pyruvic acid derivative Y

T. Nishihara, H. Nonaka, T. Naganuma, K. Ichikawa and S. Sando, Chem. Sci., 2012, 3, 800 DOI: 10.1039/C1SC00919B

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