Issue 17, 2009

Microfluidic platform for controlling the differentiation of embryoid bodies

Abstract

Embryonic stem (ES) cells are pluripotent cells, which can differentiate into any cell type. This cell type has often been implicated as an eminent source of renewable cells for tissue regeneration and cellular replacement therapies. Studies on manipulation of the various differentiation pathways have been at the forefront of research. There are many ways in which ES cells can be differentiated. One of the most common techniques is to initiate the development of embryoid bodies (EBs) by in vitro aggregation of ES cells. Thereafter, EBs can be induced to undergo differentiation into various cell lineages. In this article, we present a microfluidic platform using biocompatible materials, which is suitable for culturing EBs. The platform is based on a Y-channel device with two inlets for two different culturing media. An EB is located across both streams. Using the laminar characteristics at low Reynolds number and high Peclet numbers, we have induced cell differentiation on half of the EB while maintaining the other half in un-induced stages. The results prove the potential of using microfluidic technology for manipulation of EBs and ES cells in tissue engineering.

Graphical abstract: Microfluidic platform for controlling the differentiation of embryoid bodies

Supplementary files

Article information

Article type
Paper
Submitted
24 Feb 2009
Accepted
21 May 2009
First published
09 Jun 2009

Lab Chip, 2009,9, 2591-2595

Microfluidic platform for controlling the differentiation of embryoid bodies

W. Fung, A. Beyzavi, P. Abgrall, N. Nguyen and H. Li, Lab Chip, 2009, 9, 2591 DOI: 10.1039/B903753E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements