Raising the avermectins production in Streptomyces avermitilis by utilizing nanosecond pulsed electric fields (nsPEFs)

Avermectins, a group of anthelmintic and insecticidal agents produced from Streptomyces avermitilis, are widely used in agricultural, veterinary, and medical fields. This study presents the first report on the potential of using nanosecond pulsed electric fields (nsPEFs) to improve avermectin production in S. avermitilis. The results of colony forming units showed that 20 pulses of nsPEFs at 10 kV/cm and 20 kV/cm had a significant effect on proliferation, while 100 pulses of nsPEFs at 30 kV/cm exhibited an obvious effect on inhibition of agents. Ultraviolet spectrophotometry assay revealed that 20 pulses of nsPEFs at 15 kV/cm increased avermectin production by 42% and reduced the time for reaching a plateau in fermentation process from 7 days to 5 days. In addition, the decreased oxidation reduction potential (ORP) and increased temperature of nsPEFs-treated liquid were evidenced to be closely associated with the improved cell growth and fermentation efficiency of avermectins in S. avermitilis. More importantly, the real-time RT-PCR analysis showed that nsPEFs could remarkably enhance the expression of aveR and malE in S. avermitilis during fermentation, which are positive regulator for avermectin biosynthesis. Therefore, the nsPEFs technology presents an alternative strategy to be developed to increase avermectin output in fermentation industry.

electric fields (kV/cm), but low energy (mJ/cc) and non-thermal effects 22 . More recently, an interest is growing in concerning the proliferation effect induced by nsPEFs under relatively low electric field strength. Several researches have been reported that nsPEFs could improve the growth of Arabidopsis thaliana 23,24 and Haloxylon ammodendron seeds 25 as well as enhance the proliferation and dedifferentiation of chondrocytes 26 . Inspired by the promising capability of nsPEFs for cell proliferation enhancement, we attempt to introduce this pulsed power technology to stimulate cell growth and to increase avermectins production of S. avermitilis.
In this study, nsPEFs was applied to treat the spore suspensions of S. avermitilis. The cell viability of S. avermitilis after treatment was examined via colony forming units (CFU) count, and the morphology change of S. avermitilis was detected by scanning electron microscope (SEM). The cell growth curve and the total avermectins production of S. avermitilis were measured by UV spectrometer at 450 nm and 245 nm, respectively. Furthermore, the changes in the expression of three key genes (aveR, malE and σ 25 ) related to the avermectins production during the fermentation were analyzed by real-time RT-PCR. To better understand the mechanism of nsPEFs induced S. avermitilis proliferation enhancement, the physicochemical properties of nsPEFs-treated phosphate buffer saline (PBS) without spore were evaluated and compared by recording the oxidation reduction potential (ORP), electrical conductivity, pH and temperature.

Results and Discussions
nsPEFs device and the proposed experimental plan. As shown in Fig. 1, a self-made nsPEFs power generator was established on the basis of transmission line circuit with a pulse duration of 100 ns as previously described 13 , while the electric fields varied from 10 kV/cm to 60 kV/cm. The pulse waveforms were monitored using a digital phosphor oscilloscope (DPO4054, Tektronix, USA) equipped with a high voltage probe (P6015A, Tektronix, USA). The S. avermitilis spore suspensions were placed in 0.4 cm gap cuvette (Biosmith, aluminum plate electrodes) and then exposed to nsPEFs.
The experimental protocol for nsPEFs treatment of S. avermitilis spores is shown in Fig. 2. The cell suspension of S. avermitilis cultured in their vegetative state was spread onto solid YMS medium, and then grew in a batch  Scientific RepoRts | 6:25949 | DOI: 10.1038/srep25949 culture for 7-10 d until the transition to spores exceeded 95%. The spores were then removed and rinsed with 10 ml sterilized PBS (pH 7.0) solution to form spore suspension, which was then filtered by 4-layer sterilized gauze to remove S. avermitilis mycelia. The spore suspensions (1 ml) were treated by nsPEFs with different electric fields, while the spore suspensions without any treatment were used as the control. After nsPEFs treatment, the spores were collected, washed twice with PBS and resuspended in 1 ml PBS. Then, 1 ml spore suspensions were cultured in 50-ml Erlenmeyer flasks containing 20 ml seed medium, with glass beads, for 24 h at 28 °C on a rotary shaker (180 rpm). A 2-ml sample of the seed culture was inoculated into a 250-ml flask containing 100 ml fermentation medium. The cells were cultured for 9-11 days at 28 °C with shaking (220 rpm) to examine the yield of avermectins.
The cell viability of S. avermitilis. The S. avermitilis spore suspensions were exposed to 20 pulses of nsPEFs at 10 and 20 kV/cm, as well as 100 pulses of nsPEFs at 30 kV/cm, respectively. And the spore suspension without any treatment served as the control. The cell viability of S. avermitilis was evaluated to determine the optimum dose of nsPEFs treatment for S. avermitilis producing avermectin through CFU assay. The results in Fig. 3 show that the survival rate was 137% after 20 pulses of nsPEFs at 10 kV/cm, and proliferation rate burst to 233% for that treated by 20 pulses at 20 kV/cm. However, as the electric field intensity and the pulse number increased to 30 kV/cm and 100 pulses, the survival rate dropped to 57%. It indicates that the nsPEFs treatment with low electric field strength and less pulse number results in cell proliferation of S. avermitilis, whereas the treatment with high electric field strength and more pulse number exhibits obviously inhibition effect. This result is consistent with our previous report of nsPEFs enhancing cell proliferation of chondrocytes, in which the nsPEFs at 10 kV/cm presented a slight increase of proliferation while that cell proliferation was significantly increased at 20 kV/cm 26 . This point can also be evidenced from the work of B. Su et al. on seed growing, where the nsPEFs treatment with low intensity could elevate the germination rate of Haloxylon ammodendron seed, while high field intensity would lead to significant inhibition 25 .
The SEM pictures of S. avermitilis shown in Fig. 4 include the control, those after nsPEFs exposures of 20 pulses at 10 kV/cm and 20 kV/cm, whose surfaces are intact and smooth (Fig. 4a-c) On the other hand, the surface morphology changes significantly for the bacteria processed by 100 pulses of nsPEFs at 30 kV/cm, exhibiting obvious distortion and shrinkage of the outer layer (Fig. 4d). These different levels of morphological damage support the results of survival rate of S. avermitilis above and also prove the effects of nsPEFs on proliferation or inhibition of the cells with dependence on the electric field intensity and pulse number. Based on above results, in the following experiments we set up the e nsPEFs parameters as 5, 10 and 15 kV/cm with 20 pulses to obtain satisfied cell proliferation of S. avermitilis.
Effect of nsPEFs on cell growth of S. avermitilis. To evaluate the effects of nsPEFs on the cell growth of S. avermitilis in the long term, OD 450 were recorded every two days during an 11-day culture to obtain their cell growth curves 27 . As shown in Fig. 5a, the cell growth curve of control (black) presents an abbreviated S-shaped logistic pattern, meaning that the cells proliferated exponentially with cultivation time in the logarithmic growth phase and then reached a plateau in the stationary phase. The S. avermitilis after 20 pulses of 5 and 10 kV/cm nsPEFs treatments both show similar growth curves with the control (P > 0.05). However, since the 36 th hour, the OD 450 readings of the S. avermitilis treated with 20 pulses of nsPEFs at 15 kV/cm presented significantly higher values than that of the control, suggesting that this parameter group of nsPEFs treatment could promote cell growth of S. avermitilis, while the lower ones had little effect on its cell growth. From these results, we believe that Effect of nsPEFs on avermectin production. It has been realized that the avermectin complexes have an ultraviolet absorbance peak at 245 nm 28 . Also, Gao Hong et al. reported that the accuracy and repeatability of UV spectrophotometry for quantifying avermectin was same as that of high performance liquid chromatography (HPLC) 29 . Thus, in this study, the yields of total avermectins were detected by OD readings at 245 nm (OD 245 ) with a Rapid UV/Vis spectrometer. A calibration curve was obtained with commercial avermectin standards in the concentrations ranging from 6.4 to 64.0 μg/ml (Fig. 5c). As shown in Fig. 5b, the avermectin production of control slightly increased and reached a plateauat 10.7 μ g/ml on day 7. For the S. avermitilis treated by nsPEFs with 5 kV/cm, the avermectin production increased from 9.3 to 13.8 μ g/ml in the first 7 days, and then decreased to 11.4 μ g/ml on day 9. However, for those with 10 and 15 kV/cm nsPEFs treatments, the fermentation period of S. avermitilis to reach the highest avermectin production was shorten from 7 days to 5 days as compared to that of control and with 5 kV/cm nsPEFs treatment, and the avermectin production rose to 12.7 and 15.2 μ g/  Values are the mean ± standard deviation of three independent biological replicate experiments (n = 3). Statistical significance of differences between nsPEFs-treated and control cells is indicated with *when P < 0.05, **when P < 0.01. (b) Overall avermectin production of S. avermitilis without (control) and with nsPEFs treatment cultured in fermentation medium for 9 days. Vertical bars represent the mean ± standard deviation of three independent biological replicate experiments (n = 3). Bars labeled with different lowercase letters within the same fermentation day indicate a significant difference (P ≤ 0.05) and different uppercase letters within the same treatment indicate a significant difference (P ≤ 0.05). (c) The standard curve of avermectins.
Scientific RepoRts | 6:25949 | DOI: 10.1038/srep25949 ml, respectively, markedly higher than that of the control (10.7 μ g/ml). It should be pointed out that by recalling the results in the last section, the nsPEF treatment at 15 kV/cm could raise the efficiency of avermectin fermentation not only by reducing the fermentation period, but increasing the avermectin production as well, which may result from the enhancement of cell growth. More interestingly, even though the lower intensity treatment (5 and 10 kV/cm) produced no significant proliferation effect on cell growth of S. avermitilis (Fig. 5a), it promoted avermectin production in the long term; especially at 10 kV/cm nsPEFs treatment the fermentation period was much reduced. Therefore, it could be deduced that the contributions of nsPEFs to the improvement of avermectin production may resulted from two ways: enhancing the cell growth of S. avermitilis and regulating the avermectin biosynthesis directly.
Effect of nsPEFs on the colony appearance of S. avermitilis strains. Generally, morphology and color are two primary indicators to assess bacterial colony appearance. Thus, to determine whether nsPEFs could alter colony appearance, the S. avermitilis spores of control and treated with nsPEFs were cultivated on the solid YMS medium on day 1, 3, 5, 7 and 9 during fermentation. As shown in Fig. 6, there were no visible differences in morphology and colors between the colonies developed from the control and treated spores, indicating that the electric field intensities of 5, 10 and 15 kV/cm by nsPEFs treatment could not cause any effect on the colony appearance of S. avermitilis. It is well known that varied parameters of nsPEFs, such as duration, number of pulses and intensity, could lead to different biological processes. Intense nsPEFs treatments with high field strength could induce apoptosis in human and mouse tumor cells [30][31][32][33] , bacterial inactivation with irreversible nanopores formation 34 , and DNA damage 35 . While, mild nsPEFs treatments with low field strength could activate intracellular signaling pathways 26,[36][37][38] , improve the growth of Arabidopsis thaliana and Haloxylon ammodendron seeds, as well as enhance the cell proliferation [23][24][25][26]38 . Therefore, it is speculated that mild nsPEFs treatment in this study may not lead to the mutation of S. avermitilis. This presents a contrast result to the report from Yang et al., which suggested that S. avermitilis strains with different colony appearances giving various morphologies and colors were generated by atmospheric pressure glow discharge. High concentrations of chemically reactive species generated by plasma could destroy the mononucleotides' phosphates group in cells and accordingly resulted in a strong mutagenic effect on S. avermitilis 5 . Furthermore, to determine whether the mild nsPEFs treatments used in this study could produce S. avermitilis mutants, it is need be further studied by combining with molecular biotechnology such as various omics technologies for comparative analysis of nsPEFs-treated strains and the control strains in the future.

Evaluation of physicochemical properties of nsPEFs-treated solution. The Oxidation-Reduction
Potential (ORP), temperature and electrical conductivity of the PBS solutions without nsPEFs treatment (control) and with 5, 10 and 15 kV/cm nsPEFs treatment were all immediately measured to evaluate their physical and chemical properties. As shown in Fig. 7a, the ORP decreased almost linearly with the pulsed electric filed Figure 6. Images of S. avermitilis strains without (control) and with nsPEFs treatment grown on YMS agar during 9-day fermentation. C on the plate represents the S. avermitilis spores without any nsPEFs treatment (control); 5, 10 and 15, respectively, represent the S. avermitilis spores treated by nsPEFs with 5, 10, 15 kV/cm intensities. All pictures were obtained after the plate had been cultured for 3 days at 28 °C. . Statistical significance of differences between nsPEFs-treated and control PBS is indicated with *when P < 0.05, **when P < 0.01. intensity, changing from 61 to 40 mV, which was consistent with our previous work 25 . Recently, some studies proposed an economically competitive strategy based on ORP for improving fermentation efficiency, which suggest that ORP could serve as an index to evaluate the antibiotic production [39][40][41] . It is well known that the fermentation liquid of microorganism is generally not always in the redox equilibrium state in the fermentation process. The microorganisms absorb nutrients from the culture medium to obtain energy for cell growth and product synthesis by connecting the internal oxidation reduction reaction and intracellular metabolic processes. Some researchers have showed that ORP could regulate the microbial fermentation efficiency mainly by influencing the amount of dissolved oxygen level in the fermentation liquid. Moreover, ORP has a close relationship with the ethanol output in yeast cell 42,43 . On one hand, the trace amount of dissolved oxygen improved the cellular activity, accordingly increasing tolerance to ethanol. On the other hand, high levels of dissolved oxygen inhibited the metabolism of glycolytic pathway by acting as an electron acceptor substituted acetaldehyde, directly reducing the ethanol production. Furthermore, Lin et al. reported that ORP had a better relevance with the production of clavulanic acid than dissolved oxygen, which could increase the yield of carat clavulanic acid by 96% 44 . As a consequence, the decreased ORP has a close relationship with the improved fermentation efficiency of avermectin caused by nsPEFs. Furthermore, the temperature of PBS solution increased modestly with the pulsed electric field intensity (Fig. 7c). Accompanying with the 5, 10 and 15 kV/cm nsPEFs treatment, the temperature rose with the increasing electric field strengths, reaching approximately 20.7, 22.4 and 22.8 °C, respectively, which was similar to the results reported by Fox et al. 45 It is well known that although PEF is intended to be a non-thermal technique, a temperature rise is present due to the electric current flowing in the liquid (ohmic heating), where the pulsed electric field energy input is transformed into heat [45][46][47] . Moreover, temperature is an important basic abiotic factors affecting the bacterial production and growth. Lewis et al. reported that the growth rate of Achnanthes longipes increased significantly (P < 0.0001) with increasing temperature until the maximum growth rate was reached at 26 °C, above which growth rate dropped sharply 48 . The suitable temperature for the cell growth of S. avermitilis is 28 °C in this study, below which the increasing temperature could improve the cell growth rate. Hence, there is a possibility that the increased temperature induced by nsPEFs is closely related with the enhanced cell growth of S. avermitilis. In Fig. 7b, the electrical conductivity of nsPEFs-treated PBS was almost similar with the control (around 5.25), indicating that electrical conductivity have no significant contribution to the cell growth and avermectin production of S. avermitilis.

Effect of nsPEFs on expression of aveR, malE and σ 25 . The entire genome sequence of S. avermitilis,
including that of the gene cluster for the biosynthesis of avermectin, has already been determined 49,50 , and fundamental studies on the biosynthetic pathway of avermectins and their regulatory mechanism have also been conducted 2,51 . Some studies on functional genes and global regulatory genes, as well as on gene manipulation of pertaining to the biosynthesis of avermectins in S. avermitilis have been carried out 51 . In this study, three important genes, aveR, malE and σ 25 , associated with avermectin production of S. avermitilis, were analyzed by real-time RT-PCR tests. The avermectin gene cluster spans a distance of 82 kb and has a putative pathway-specific regulatory gene, aveR 49 , which encodes a pathway-specific activator essential for avermectin biosynthesis as well as a negative regulator for oligomycin biosynthesis 52 . Moreover, malEFG-a encodes proteins involved in the maltose transport system, and plays an essential role in avermectins production in S. avermitilis. Overexpression of malEFG-a in S. avermitilis enhanced the utilization rate of starch, resulting in increased antibiotic production and reduced fermentation periods 27 . In addition, σ 25 is an extracytoplasmic function (ECF) σ factor in S. avermitilis that plays a differential regulatory role in avermectin and oligomycin biosynthesis. Gene deletion, complementation, and overexpression experiments showed that σ 25 could indirectly inhibit avermectins production by affecting the transcription of the pathway-specific activator gene aveR 53 .
The result of Fig. 8a,b shows that the transcript levels of aveR and malE were significantly higher for the S. avermitilis strains treated by nsPEFs at 15 kV/cm than that of control on day 3 and day 5, indicating that nsPEFs could enhance the expression of aveR and malE. While, the transcript level of σ 25 was almost the same without difference between the nsPEFs-treated group and the control (P > 0.05), suggesting that nsPEFs has no effect on the expression of σ 25 during the fermentation process (Fig. 8c).
It was generally accepted that nsPEFs provoke a remarkable increase in cytoplasmic Ca 2+ , which presumably arises from the cumulative effects of nanopore formation and direct actions of nsPEFs on intracellular Ca 2+ stores and various Ca 2+ channels in the cell membrane 20,[54][55][56] . Calcium and calcium-binding proteins including those resembling calmodulin are implicated in numerous diverse processes in bacteria including chemotaxis, sporulation, virulence, the transport of sugars and proteins, phosphorylation, heat shock, the initiation of DNA replication, septation, nucleoid structure, nuclease activity and recombination, the stability of envelope, and phospholipid synthesis and configuration 57 . Moreover, calcium ions could also serve as second messengers and provide important upstream signals for cellular mechanisms to occur such as proliferation, differentiation and apoptosis 58 . Therefore, recently, many researchers have started to pay attention on the specific signaling events provoked in response to Ca 2+ mobilization by nsPEFs. K. Morotomi-Yano et al. provided experimental evidence that nsPEFs-induced Ca 2+ mobilization directly affects the intracellular signal pathway involving calcium/ calmodulin-dependent protein kinase kinase (CaMKK) and AMP-activated protein kinase (AMPK) in human cells 59 . G. P. Tolstykh et al. reported that exposing cells to nanosecond pulsed electric fields causes a rapid increase in intracellular calcium, enabling a pathway that activates protein kinase C (PKC) for various physiological functions, including hormone secretion, action potentials (AP) propagation, and muscle contraction 60 . Kun Zhang et al. also reported that nsPEFs could enhance the proliferation and dedifferentiation of chondrocytes via activation of the wnt/β -catenin signaling pathways accompanied by the bursts of intracellular calcium 26 .
Taken together, it is speculated that nsPEFs may induce calcium ion influx in S. avermitilis cells, the extent of which depends on nsPEFs dosage, set off a cascade of signaling events that dictate cellular response and may ultimately elevate the expression of aveR and malE, consequently resulting in the raising of avermectins production in S. avermitilis. Additionally, given the fact that the avermectins biosynthesis in S. avermitilis is too complex to understand clearly, future study will investigate the effects of nsPEFs on the complex metabolic pathways and global regulation networks of avermectins biosynthesis in S. avermitilis via molecular biotechnologies such as genomics, proteomics and metabolomics, which will eventually contribute to the creation of a powerful S. avermitilis strain for avermectins production.
In conclusion, the results in this study show that nsPEFs can significantly improve avermectins production by prompting cell growth and enhancing expression of gene associated closely with avermectin biosynthesis in S. avermitilis. As an alternative strategy, nsPEF could provide a potentially feasible way in avermectins fermentation.

Methods
Strain, media and culture conditions. The strain used in this work was S. avermitilis ATCC31267 (wildtype; avermectin-producer) 61 , which was grown at 28 °C on a solid YMS (yeast extract-malt extract-starch) medium for sporulation 62  . Subsequently, they were split longitudinally, dried naturally, fixed and sputter-coated with gold-palladium for examining with SEM (S-4800, HITACHI, Japan). Moreover, the images of S. avermitilis spores of control and nsPEFs treatment grown on the solid YMS medium on day 1, 3, 5, 7 and 9 during fermentation were obtained after the plate had been cultured for 3 days at 28 °C. Fermentation and analysis of avermectin production. After the nsPEFs-treated S. avermitilis cultured in the fermentation medium for 9 days (mentioned above), 5 ml of the fermentation broth was sampled on day 1, 3, 5, 7 and 9 from every flask and centrifuged at 5000 g for 5 min. The precipitate was collected, and 2 ml acetone was added and shaken for 30 min. Subsequently, 3 ml ethyl acetate was added to the mixture and shaken for an additional 30 min. After the mixture was left standing for 10 min, 50 μ l of the supernatant was then diluted with 2 ml methanol. The yields of total avermectins were detected by OD readings at 245 nm (OD 245 ) with a Rapid UV/ Vis spectrometer 29 , using authentic samples of avermectin as internal standards.
Physicochemical properties measurement. Oxidation-reduction potential (ORP), electrical conductivity, and temperature of PBS were immediately measured after nsPEFs treatment. ORP and temperature were measured by a multimeter pH & Redox (Mettler-Toledo, Switzerland). And electrical conductivity was measured with electric conductivity meter (DDB-303A).
RNA preparation and real-time RT-PCR analysis. After grown in soluble FM-II for 3 and 5 days, mycelia of S. avermitilis were collected, flash-frozen in liquid nitrogen, and ground into fine powder. Total RNA was isolated from the ground mycelial paste using Trizol reagent (Invitrogen, USA) according to the manufacturer's instructions, and the quality of RNAs were assessed using a NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). Then, the sample was treated with DNase I to remove the contaminating chromosomal DNA. The treated RNA sample (2 μ g) was reverse transcribed using M-MLV (RNase H − , TaKaRa), random hexamers (25 μ M), and a dNTP mixture (10 mM each). Real-time RT-PCR analysis was performed to determine the transcription levels of aveR, malE and σ 25 using the obtained cDNA as template and with the gene-specific primers listed below: for aveR, 5′ -CCGCGACTTCCTCACCG-3′ (sense) and 5′ -GGACTCGCTCAGCAG-3′ (antisense); for malE, 5′ -TACGCCCACATCCAGGACG-3′ (sense) and 5′ -GTCGGCAGCG TGGAGTTC-3′ (antisense); for σ 25 , 5′ -CTGCAGAGCGCGCTGTTC-3′ (sense) and 5′ -GGTTGGTCATGGTGCGACG-3′ (antisense); for hrdB, 5′ -TACTGCGCAGCCTCAACCAG-3′ (sense) and 5′ -GCCGATCTGCTTGAGGTAGTC-3′ (antisense). The RT-PCR experiments were performed using FastStart Universal SYBR Green Master (ROX; Roche, USA) with analysis by an ABI 7900HI Sequence Detection System (Applied Biosystems, USA) using optical-grade 96-well plates. Template cDNA, 10 μ l SYBR Green Master, and forward and reverse primers (each 300 nM) were mixed in each reaction system (total volume 20 μ l). The PCR protocol consisted of 95 °C for 10 min, 40 cycles of 95 °C for 10 s, and 60 °C for 30 s with a single fluorescence measurement. For semi-quantitative analysis, transcript of aveR, malE and σ 25 was normalized to that of hrdB, which encodes the major sigma factor in Streptomyces, was used as positive control for RT-PCR assay. RT-PCR without the initial reverse transcription step was carried out as a negative control for all analyses to confirm the absence of DNA contamination. Data were verified in three independent experiments. Statistical analysis. All data were obtained from three independent biological replicate experiments (n = 3).
The values from all experiments were expresses as the mean ± standard deviation (SD). Statistical analysis was performed using SPSS statistical package 17.0 (SPSS Inc., USA). An analysis of variance (ANOVA) was conducted to compare the effects of different nsPEFs treatments on the avermectin production during formation process, and significant differences between the mean values were identified by the Student-Newman-Keul's multiple range test with a confidence level at P ≤ 0.05. Moreover, the paired-sample t-test was applied to evaluate the statistical significance of differences between the nsPEFs-treated and control groups on the cell viability, cell growth and gene expression of S. avermitilis, as well as the physicochemical properties (ORP, electrical conductivity, pH and temperature) of PBS. The difference is expressed as *P < 0.05, **P < 0.01, and ***P < 0.001.