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Requirement of the coiled-coil domains of p92c-Fes for nuclear localization in myeloid cells upon induction of differentiation

Oncogene volume 22, pages 1712–1723 (2003)Cite this article

Abstract

The nonreceptor tyrosine kinase Fes is implicated in myeloid cells differentiation. It has been observed that its localization can be cytoplasmic, perinuclear, or nuclear. To further characterize this point, we studied Fes subcellular localization in myeloid cell lines (HL60 and K562) and in COS1 cells. Fes was observed in both the nucleus and the cytoplasm of HL60, K562 cells overexpressing Fes and only in the cytoplasm of COS1 cells, suggesting that nuclear localization is cell context dependent. Moreover, in myeloid cells, the treatment with differentiation-inducing agents such as retinoic acid, phorbol esters and vitamin D, is followed by an increase of the oligomeric form of Fes in the nucleus. In fact, oligomerization seems to be necessary for translocation to occur, since Fes mutants missing the coiled-coil domains are not able to form oligomers and fail to localize in the nucleus. The active form of Fes is tyrosine phosphorylated; however, phosphorylation is not required for Fes to localize in the nucleus, since tyrosine kinase inhibitors do not block the translocation process.

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Authors and Affiliations

  1. Dipartimento di Scienze Biomediche, Università di Modena e Reggio Emilia, Sezione di Chimica Biologica, Via Campi, 287, Modena, 41100, Italy

    Enrico Tagliafico, Michela Siena, Tommaso Zanocco-Marani, Rossella Manfredini, Elena Tenedini, Monica Montanari, Alexis Grande & Sergio Ferrari

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  1. Enrico Tagliafico
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  2. Michela Siena
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Correspondence to Sergio Ferrari.

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Tagliafico, E., Siena, M., Zanocco-Marani, T. et al. Requirement of the coiled-coil domains of p92c-Fes for nuclear localization in myeloid cells upon induction of differentiation. Oncogene 22, 1712–1723 (2003). https://doi.org/10.1038/sj.onc.1206279

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