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Inactivation of the RAD51 recombination pathway stimulates UV-induced mutagenesis in mammalian cells

Oncogene volume 21, pages 4065–4069 (2002)Cite this article

Abstract

We have examined the impact of the RAD51 recombination pathway on recombination and mutagenesis induced by UV-C in mammalian cells. We used hamster CHO cell lines that express different forms of Rad51 protein, resulting in stimulation or inhibition of spontaneous gene conversion. Spontaneous mutagenesis was affected by none of the RAD51 forms. The wild-type mouse MmRAD51 affects neither UV-induced recombination nor UV-induced mutagenesis. In contrast, the dominant negative SMRAD51 strongly impairs UV-induced recombination while it stimulates UV-induced mutagenesis. Our results show that a defect in the RAD51 gene conversion pathway reveals (a) mutagenic alternative pathway(s) to repair UV-damage, in mammalian cells.

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Acknowledgements

Thanks are due to Dr M Jasin for providing the CHO-DRA10 cell line. We thank Drs P Bertrand and D Marsh for helpful discussion and critical reading of the manuscript. Sarah Lambert is supported by a fellowship from ‘La Ligue Nationale Française contre le Cancer’. This work was supported by Electricité de France, ANRS and ARC (9822).

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  1. Département de Radiobiologie et Radiopathologie, UMR217 CNRS-CEA, CEA, Direction des Sciences du Vivant, 60-68 avenue du Général Leclerc, Fontenay aux Roses, 92 265, cedex, France

    Sarah Lambert & Bernard S Lopez

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  1. Sarah Lambert
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  2. Bernard S Lopez
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Correspondence to Bernard S Lopez.

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Lambert, S., Lopez, B. Inactivation of the RAD51 recombination pathway stimulates UV-induced mutagenesis in mammalian cells. Oncogene 21, 4065–4069 (2002). https://doi.org/10.1038/sj.onc.1205535

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